精氨酸多肽-抗生素偶联物对细菌性膀胱炎家兔动物模型治疗的研究

Therapeutic effect of antibiotic conjugated with 11 poly-arginine peptide on rabbit model with bacterial cystitis

目的:探讨精氨酸多肽-环丙沙星偶联物(R11-Cipro)对细菌性膀胱炎家兔模型的治疗作用。方法:选取4月龄新西兰家兔50只,成功建立细菌性膀胱炎动物模型后,随机分为5组。A组为空白对照组、B组为膀胱灌注R11组、C组为膀胱灌注Cipro组、D组为膀胱灌注Cipro-R11组、E组为经耳缘静脉注射Cipro组。观察排尿频率、尿液细菌培养以及获取膀胱组织,HE染色,观察其病理变化。结果:建模后膀胱黏膜上皮出现严重的炎症改变和炎症细胞浸润,R11-Cipro组治疗后膀胱炎症改善最明显,甚至优于经静脉Cipro组(P<0.05);在排尿频率及尿细菌培养方面,R11-Cipro组亦显著优于其余四组。结论:R11-Cipro经膀胱灌注对家兔细菌性膀胱炎具有显著的治疗效果,R11这个高效载体能有效运载抗生素到膀胱内发挥良好的靶向杀菌活性。

Objective To investigate the therapeutic effect of ciprofloxacin （Cipro） conjugated with 11poly-arginine peptide （Rll） on rabbit model with bacterial cystitis （BC）. Methods 50 New Zealand rabbits of4-month old were chosen to establish the models and evenly divided into 5 groups randomly Group A： normalcontrol; Group B： intravesical instillation （II） of Rll; Group C： II of Cipro; Group D： II of Rll-Cipro; GroupE： intravenous injection of Cipro. Several parameters were observed which included： urinary frequency, positiverate of urine culture, histopathological analysis of cystitis stained with hematoxylin and eosin. Results Severeinflammatory responses and massive infiltration of inflammatory cells were observed after the models wereestablished. Rll-Cipro group was better than intravenous injection of Cipro group in treating cystitis （P 〈 0.05）.Ri 1-Cipro group was better than the other four groups in urinary frequency and urine culture. ConclusionsIntravesical instillation of R11-Cipro demonstrated significant therapeutic effect on bacterial cystitis. R11, as anefficient vector, could deliver specific antibiotics to bladder mucosa precisely and function well locally.