慢性心力衰竭患者外周血白介素-27与树突状细胞亚群变化的研究

Changes in the Levels of IL-27 and dendritic cell sub-population in peripheral blood of patients with chronic heart failure

目的探讨白介素-27(IL-27)、树突状细胞(dendritic cells,DCs)在慢性心力衰竭患者外周血中的表达变化。方法选取2012年6月至2013年12月入住南方医科大学珠江医院心血管内科的慢性心力衰竭患者96例,根据纽约心功能分级,分为纽约心功能(NYHA) Ⅳ级组、NYHA Ⅲ级组、NYHA Ⅱ级组以及NYHAⅠ级组,心脏超声检测心功能变化,流式细胞术检测外周血DCs亚群比例和计数,ELISA法检测外周血IL-27、BNP的表达。结果 NYHA Ⅰ级组、NYHA Ⅱ级组、NYHA Ⅲ级组、NYHA Ⅳ级组,IL-27浓度呈依次递减(P<0.05),BNP浓度依次递增;NYHAⅣ级组和NYHA Ⅲ级组的mDC比例和mDC计数明显低于NYHAⅠ级组和NYHA Ⅱ级组(P<0.05)。结论IL-27和树突状细胞(dendritic cells,DCs)参与了慢性心力衰竭的发生发展。

Objective To investigate the level changes of IL-27 and dendritic cells （DCs） in patients with chronic heart failure. Methods A total of 96 patients with chronic heart failure were enrolled from June 2012 to December 2013 in Zhu- jiang Hospital Affiliated to Southern Medical University and then divided into NYHA Ⅳ group, NYHA m group, NYHA Ⅱ group and NYHA I group according to the New York Heart Association Classification. Heart function were determined by cardiac ultrasound;the ratio and number of DC sub-populations in peripheral blood were determined by flow eytometry;and the levels of IL-27 and B-type natriuretic peptide （BNP） were determined by ELISA. Results The levels of IL-27 were decreasing but BNP were increasing orderly from NYHA Ⅰ group,NYHA Ⅱ group,NYI-IA Ⅲ group and NYHA IV group （P 〈 0. 05）. The proportion and count of mDCs were lower in NYHA m group and NYHA Ⅳ group than those in NYHA I group and NYHA Ⅱ group （P 〈 0. 05）. Conclusion IL-27 and DCs participate in the development of chronic heart fail-