黄秋葵果实粉对衰老模型小鼠抗氧化能力的影响

Effects of Okra Fruit Powder on Antioxidant Abilities in Aged Mice Induced by D-galactose

探讨黄秋葵果实粉(OFP)对D-半乳糖致衰老模型小鼠抗氧化能力的影响。以昆明种小鼠为研究对象,颈背部皮下注射D-半乳糖造模。正常对照组、模型对照组饲喂基础饲料,实验组小鼠饲喂含受试物低、中、高剂量(32.00、64.00、128.00 g/kg)饲料,实验期56 d,测定血清和肝组织匀浆中超氧化物岐化酶(SOD)、谷胱苷肽过氧化物酶(GSHPx)、过氧化氢酶(CAT)的活性及丙二醛(MDA)的含量。D-半乳糖致衰小鼠SOD活性、GSH-Px活性和CAT活性均明显下降,MDA含量明显上升,与正常小鼠比较差异显著;饲喂不同剂量的黄秋葵果实粉后均能提高小鼠血清、肝脏组织中的SOD、GSH-Px和CAT活性,同时降低血清、肝脏组织中MDA含量。适量摄入黄秋葵果实粉能显著提高衰老小鼠的抗氧化能力,具有一定延缓衰老的作用。

To investigate the effects of Okra fruit powder （OFP） on antioxidant abilities in aged mice induced by D-galactose. The subacute aged model mice were made by neck back subcutaneous injection of D-galactose continually. The mice were given different doses of Okra fruit powder 32.00,64.00 g/kg and 128.00 g/kg fed by diet for 56 d respectively.The content of malondialdehyde （MDA）and activity of superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX） and catalase （CAT） in the serum and liver of the mice were tested. The SOD, GSH-Px and CAT activities in aged model group were significantly decreased and MDA significantly increased compared with normal mice;OFP in different doses evidently increased the SOD, GSH-Px and CAT activities and reduced the MDA level in serum and liver in mice. OFP can obviously increase the antioxidant ability of mice.