建立数学模型优化冬凌草中熊果酸提取工艺

A Mathematical Model is Established to Optimize Extraction Technology of Ursolic Acid in Rabdosia Rubescens

研究采用超高压提取冬凌草中熊果酸的最佳工艺条件,并且同回流提取法和超声提取法进行比较。在单因素实验的基础上,建立L(93)4正交实验的数学模型对超高压提取冬凌草中熊果酸的工艺进行优化,以熊果酸的得率为指标,考察料液比(g∶mL)、乙醇浓度、超高压压力、加压时间对熊果酸得率的影响。得到优化的工艺条件为:固液比1∶22、乙醇浓度75%、超高压压力320MPa,超高压时间4min,该条件下熊果酸的得率可达0.322%。超高压提取熊果酸得率高,提取时间短,是一种提取冬凌草中熊果酸的适宜方法。

To optimum of extracting ursolic acid from Rabdosia rubescens （Hemsl.）Hara. Ultra high pressure （UHP） method was studied and was compared to reflux extraction method and ultrasonic wave extraction method. According to the signal factor test, the L9（3^4） orthogonal design was used to optimum extraction conditions. Using with extraction yield of ursolic acid as index, the influence of solid to liquid ratio, ethanol concentration,UHP pressure, UHP time were studied. Optimum extraction conditions were as follows： solid to liquid ratio 1 ： 22 （g： mL）, ethanol 75 %, UHP pressure 320 MPa and UHP time 4 min, The yield of UA was 0.322 %. The UHP extraction method was a suitable method for it has higher yield and shorter extraction time.