大黄酚对脑缺血再灌注小鼠心脏组织过氧化氢及过氧化氢酶的影响

Effects of chrysophanol on hydrogen peroxide and catalase in cerebral ischemia reperfusion mice heart tissue

目的研究大黄酚(chrysophanol,Chry)对脑缺血再灌注(IR)后小鼠心脏组织中过氧化氢(H2O2)及过氧化氢酶(CAT)的影响,探讨大黄酚对脑缺血再灌注后对心脏损伤的保护作用机制。方法取昆明种小鼠80只,3.5%水合氯醛麻醉后,制备小鼠IR模型。将造模成功的74只小鼠随机分为5组,药物组(IR+Chry)45只,分为高、中、低剂量3个组,各15只,模型组15只,假手术组14只。药物组于缺血前30 min分别腹腔注射大黄酚10.0、1.0、0.1 mg/kg;模型组于缺血前30 min腹腔注射溶剂10 mL/kg;假手术组只进行手术,但不进行IR,腹腔注射溶剂10 mL/kg。模型组和药物组小鼠恢复血供后10 min快速断头处死,取心脏组织,制成10%心脏组织匀浆,测定小鼠心脏组织中H2O2含量和CAT活力。结果与假手术组比较模型组小鼠心脏组织内H2O2含量明显增加﹙P<0.05),心脏组织内CAT活力明显降低﹙P<0.05);与模型组比较高剂量大黄酚组、中剂量大黄酚组小鼠心脏组织内H2O2含量明显减少(P<0.01)。与模型组比较高剂量大黄酚组小鼠心脏组织内CAT活力明显提高(P<0.01)。结论大黄酚对脑IR心脏损伤的保护作用可能通过提高心脏组织中CAT活力起作用。

Objective To study the effects of chrysophanol on hydrogen peroxide and catalase in cerebral ischemia reperfusion mice heart tissue and further analyze the related mechanisms of protective effects on heart injury induced by cerebral ischemia reperfusion. Methods Cerebral ischemia reperfusion models of mice were prepared with 90 kunming mice anesthetized by 3.5% chloral hydrate. The models of 74 mice that prepared successfully were randomly divided into five groups： sham -operated group（ 14 mice, operate but not do cerebral ischemia reperfusion ）, model group（ I - R, 15 mice, operate and do cerebral ischemia reperfusion）, high dose chrysophanol group（ I - R ＋ Chry, 15 mice, ip 10 mg/kg）, middle dose chrysophanol group（I - R ＋ Chry, 15 mice, ip 1 mg/kg）, low dose chrysophanol group（ I - R ＋ Chry, 15 mice, ip 0.1 mg/kg）. Mice of model group and chrysophanol group were be- headed quickly when blood flow is restored after ten minutes, and then heart tissues were taken out of beheaded mice to make 10% heart homogenate. Content of H2O2 and CAT activities in heart were measured. Results Compared with control group H2O2 content in mice heart tissue of model group increased significantly, CAT activity obviously reduced in the heart tissue, the difference was statistically significant （ P 〈0.05） ; Compared with model group, H202 content in the heart tissue of mice of high dose chrysophanol group and middle dose chrysophanol group decreased significantly, the difference was statistically significant （Chry 10.0, Chry 1.0 mg/kg, P 〈 0.01 ）. Compared with model group CAT activities of heart in high dose ehrysophanol group increased significantly, the difference was statistically significant （Chry 10.0 mg/kg, P 〈 0. 01 ）. Conclusion Protective effects of chrysophanol on heart injury of cerebral ischemia reperfusion can be affected by increasing the activity of CAT in heart tissue.