小麦SOD基因的电子克隆及生物信息学分析

Genes in silico cloning and bioinformatics analysis of SOD in common wheat

以NCBI中的EST数据库为主要数据来源,以一系列生物信息学软件为工具,进行了超氧化物歧化酶(SOD)基因的电子克隆及生物信息学分析.结果表明:通过电子克隆方法获得了SOD基因的编码区全长序列,该预测的SOD蛋白质氨基酸序列与数据库中同类基因的蛋白质氨基酸序列具有极高的相似性,并且含有完整的保守结构域;电子克隆到的SOD基因编码的不是分泌蛋白,也不是膜蛋白,而是胞质蛋白.通过某个基因的一个EST序列采用电子克隆的手段进行基因全长的电子拼接是可行的.

Taking EST database in the NCBI as the main data source,with a series of bioinformatics software as tools,the SOD gene in silico cloning and bioinformatics analysis was been done.The results showed that obtained SOD genes coding region of full-length sequence through in silico cloning.The prediction amino acid sequence of SOD protein has a very high sequence similarity with the protein amino acid of similarity genes in the protein database of NCBI,and contains a complete conservative domain structure.The encoding protein of SOD gene obtained in this study by in silico cloning is not secreted proteins,membrane protein,but the cytoplasmic protein.Gene full-length stitching of encoding region is feasible by means of in silico cloning through a relative EST sequence.