聚己内酯(PCL)多孔亚微米纤维的制备

Fabrication of Poly (ε-caprolactone) Porous Sub-microfibers by Electrospinning

以DMF、丙酮、二氯甲烷和丙酮/二氯甲烷为溶剂,通过静电纺丝方法制备PCL亚微米纤维.通过SEM观察纤维形貌及其孔径大小,通过N2吸附-脱附曲线计算纤维的比表面积,通过拉伸实验测试纤维的力学性能.实验结果表明:以单一DMF、丙酮为溶剂制备得到表面光滑无孔亚微米纤维.而以二氯甲烷为溶剂无法制备连续的纤维.以丙酮/二氯甲烷为溶剂制备得到直径为0.86±0.14μm、孔径大小为65.0±17.5nm,比表面积为21.15m2/g的亚微米纤维.相比较无孔纤维,多孔PCL亚微米纤维的拉伸强度和杨氏模量略有下降,而断裂伸长率有所增加,主要是因为拉伸过程中,多孔纤维发生一定程度的收缩.PCL亚微米纤维表面的多孔结构有利于细胞的黏附与生长,因而有望作为组织工程支架材料.

PCL sub--microfibers were prepared by electrospinning using DMF, acetone, dichlorometha- neas and acetone/ dichloromethaneas as solvent. The fiber diameter and surface morphology was studied using scanning electron microscopy （SEM）, specific surface area was calculated by N2 adsorption/desorp- tion isotherms and mechanism property was measured by tensile experiment. The results show that the sub--microfibers with smooth surface were prepared using DMF or acetone as single solvent, and spinning was very difficult using dichloromethaneas as solvent. However, PCL porous sub--microfibers with diam- eter of 0.86±0.14 μm, mesopores of 65.0±17.5 nm and specific surface area of 21.15 m2/g were pre- pared using acetone/dichloromethaneas as solvent. Compared with PCL sub--microfibers, tensile strength and Young＇s modulus of PCL porous sub--microfibers were slightly decreased, and the elongation at break was increased. This was attributed to the pore was shrunk during drawing. Due to the porous struc- ture, this special PCL fibrous membrane would be in favor of cell adhesion and growth, so they are expec- ted to be a very promising tissue engineering scaffold.