裸花紫珠试管苗生产技术初探

Production Technologies of Callicarpa nudiflora Hook. et Arn. Test-Tube Plantlets

以带腋芽的裸花紫珠（Callicarpa nudiflora Hook.et Arn.）嫩茎段为外植体,研究其组培快繁技术.结果表明,用0.1％的升汞处理外植体15 min,接种于MS＋1.5 mg/L 6-BA＋30 g/L蔗糖培养基上,7d后萌发腋芽;丛生芽在MS＋2.0 mg/L 6-BA＋30 g/L蔗糖的培养基增殖效果最佳,增殖系数达8.50;丛生芽的继代培养周期是30 d,超出40 d时会有死株现象;培养基MS＋0.5～0.8 mg/L NAA＋0.5 g/L活性炭＋30 g/L蔗糖适宜诱导生根,生根率达100％;生根苗移栽在V河沙∶V木屑∶V园土=1∶1∶1的混合基质中,塑料薄膜保湿20 d成活率达93％,且植株生长良好.

Using tender stem segments of Callicarpa nudiflora as explants,the techniques of tissue culture and rapid propagation in vitro were studied.Results showed that the optimum disinfector was 0.1％ HgCl2 for 15 min,with MS＋6-BA1.5 mg/L＋ sucrose 30 g/L as the initial medium.After inoculation for 7 d,the axillaries buds began to germinate.Using MS＋6BA 2.0 mg/L ＋ sucrose 30 g/L as the proliferation medium,the proliferation multiples reached 8.50.The best cycle of subculture training was 30 d.The plantlets would appear dead when more than 40 d.The rooting culture medium was MS＋ NAA 0.5～0.8 mg/L＋ active carbon 0.5 g/L ＋ sucrose 30 g/L,with the best rooting effect and the rate of rooting of 100％.When the rooted seedlings were transplanted into the mediums of sand∶soil∶sawdust （1∶1∶1） and covered with plastic film for keeping moisture for 20 d,the survival rate reached up to 93％.