新疆某猪场猪源耐药大肠杆菌β-内酰胺酶及16SrRNA甲基化酶检测及分析

Detection and Analysis of β-lactamase and 16S rRNA methylase in Resistant Escherichia coli Isolates from A Hoggery in Xinjiang

【目的】了解新疆乌市某猪场猪源耐药大肠杆菌携带β-内酰胺酶和16S rRNA甲基化酶基因及其共存情况。【方法】采用PCR方法检测203株β-内酰胺类药物(包括头孢噻呋、阿莫西林/克拉维酸、氨苄西林)多药耐药大肠杆菌进行β-内酰胺酶及16S rRNA甲基化酶基因,并测序检出基因的PCR产物。【结果】检出83株菌携带bla_(TEM)(40.89%),2株菌携带bla_(CTX-M)(0.99%);检出2株菌携带rmtB(0.99%)基因,并且同时携带bla_(TEM)未检出其它被检耐药基因。【结论】该猪场猪源大肠杆菌中存在β-内酰胺酶及16S rRNA甲基化酶介导的耐药,并且共存。β-内酰胺酶的基因型以bla_(TEM)为主;16S rPdNA甲基化酶检出率较低。猪源大肠杆菌存在对头孢菌素类和氨基糖苷类抗生素多重耐药的风险,有必要对此猪场耐药基因流行情况进行密切监测。

[Objective]To research the situation of the resistant Escherichia coli carrying β-lactamases and / or 16S rRNA methylase gene and its coexistence conditions in a pig farm in Xinjiang.[Method] β-lactamase gene （including ceftiofur,amoxicillin / Clavulanic acid,ampicillin） and 16S rRNA methylase gene in 203 isolates multidrug resistance Escherichia coli were detected by PCR.Those products of PCR were analyzed by sequencing.[Result] The results of the test were：83 strains carTied blaTEM （40.89％） gene,two strains carried blaCTX_M（O.99％） ; the two other strains carried rmtB （0.99％） genes,and at the same carried blaTEM genes,and other resistance genotypes were not seized.[Conclusion] β-lactamase and 16S rRNA methylase-mediated resistance existed and coexisted in Escherichia coli from the pig far in Xinjiang,β-lactamase blaTEM genotype was the dominant one,with a lower rate of 16S rRNA methylase.The presence of Escherichia coli from pigs displayed risks of muhi-durg resistence to cephalosporins and aminoglycosides so it is necessry for this far to closely monitor the prevalence of drug resistance genes.