银杏黄酮对非酒精性脂肪肝病小鼠肝脏NF-κBp65和TNF-α的影响

Effects of gingko flavonoid on expression of NF-κBp65 and TNF-αin mice with non-alcoholic fatty liver disease

目的观察不同剂量银杏黄酮对非酒精性脂肪肝病(NAFLD)小鼠肝脏核因子-κBp65(NF-κBp65)和肿瘤坏死因子(TNF-α)的影响。方法采用高脂饲料喂养4周,构建小鼠NAFLD模型。小鼠随机分为正常对照组,高脂模型组,银杏黄酮高、中、低剂量治疗组。银杏黄酮高、中、低剂量组分别予不同剂量银杏黄酮灌胃,正常对照组和高脂模型组予生理盐水灌胃。治疗12周后处死小鼠检测肝组织NF-κBp65表达,血清TNF-α、三酰甘油(TG)水平。结果与正常对照组比较,高脂模型组NF-κBp65、TNF-α、TG水平升高(P<0.05)。与高脂模型组比较,银杏黄酮高、中、低剂量组NF-κBp65、TNF-α、TG水平降低(P<0.05),且剂量越高降低越明显。结论银杏黄酮可减少NF-κBp65及TNF-α、TG的产生,从而对NAFLD起到一定治疗作用。

Objective To investigate the effects of different doses of gingko flavonoid on the expression of nuclear factor-κBp65 （NF-κBp65） and tumor necrosis factor-α（TNF-α） in mice with non-alcoholic fatty liver disease（NAFLD） .Methods The mice mod-el of NAFLD was constructed by the high fat diet for 4 weeks .The mice were randomly divided into the control group ,high fat model group ,high ,middle and low doses of gingko flavonoid groups .High ,middle and low doses of gingko flavonoid groups were respectively gavaged with the different doses of gingko flavonoid .The normal control group and the high fat model group were ga-vaged with normal saline .After 12 weeks ,the mice were killed for taking liver tissue and detecting NF-κBp65 expressions ,and ser-um TNF-αand triacylglycerol（TG） levels were detected .Results Compared with the normal control group ,the levels of NF-κBp65 ,TNF-αand TG in the high fat model group were increased（P〈0 .05） .Compared with the high fat model group ,the levels of NF-κBp65 ,TNF-αand TG in the high ,middle and low doses of gingko flavonoid groups were decreased （P〈0 .05） ,moreover the higher the dose ,the more obvious the decrease .Conclusion Gingko flavonoid may play certain role in the treatment of NAFLD by reducing the generation of NF-κBp65 ,TNF-αand TG .