中性粒细胞弹性蛋白酶抑制剂对颅脑创伤大鼠血脑屏障通透性和脑水肿的影响

Research on effect of neutrophil elastase inhibitor on blood-brain barrier permeability and cerebral edema in TBI rats

目的探讨中性粒细胞弹性蛋白酶(NE)抑制剂对颅脑创伤(TBI)大鼠血脑屏障(BBB)通透性改变和脑水肿的影响。方法将99只大鼠分为对照组、TBI组和干预组(分为6、24、48、72、168h亚组),复制大鼠液压打击模型,干预组给予西维来司钠,分别测定各组脑组织NE的浓度、BBB通透性和脑组织含水率,并进行比较分析。结果 TBI组和干预组大鼠脑组织NE浓度、BBB通透性和脑组织含水率在各时间点都高于对照组,干预组的NE浓度在24、48、72、168h低于TBI组,BBB通透性和脑组织含水率在48、72、168h低于TBI组(P<0.05)。结论西维来司钠能抑制TBI大鼠脑组织NE的释放,降低BBB的通透性并减轻脑水肿发生,表明西维来司钠对大鼠TBI继发性损伤有保护作用。

Objective To investigate the effect of neutrophil elastase （NE） inhibitor on the blood-brain barrier（BBB） permeabili-ty and hydrocephalus in rats with traumatic brain injury （TBI） .Methods 99 SD rats were randomly divided into the control group , the TBI group and the intervention group（dividing into 5 sub-groups：6 ,24 ,48 ,72 ,168 h） .The hydraulic impact model of rats was duplicated .Sivelestat sodium was given in the intervention group .The NE concentration in the brain tissue ,BBB permeability and brain water content were detected in each group and performed the comparative analysis .Results The NE concentration in the brain tissue ,BBB permeability and brain water content at each timepoint in the TBI group and the intervention groups were higher than those in the control group .The NE concentration at 24 ,48 ,72 ,168 h in the intervention group was lower than that in the TBI group .The BBB permeability and the brain water content at 48 ,72 ,168 h in the intervention group were lower than those in the TBI group（P〈0 .05） .Conclusion Sivelestat sodium can inhibit the NE release in TBI rat brain tissue ,reduce the BBB permeability and the occurrence of hydrocephalus ,which indicating that sivelestat sodium has the protective effect on TBI secondary lesion in rat .