不同来源致耐受DC对大鼠牙移植后牙根吸收及愈合影响的研究

Effect of different sources of tolerogenic dendritic cells on root resorption and healing after tooth transplantation in rats

目的探讨不同来源致耐受树突细胞(DC)对大鼠同种异体牙移植后牙根吸收及愈合的影响。方法用BN、Lewis大鼠分别作为供、受体建立动物实验模型,采用完全随机法分为4组,每组20只:A为同基因移植;B、C、D为同种异体移植;A、B组于术前7d给大鼠输注PBS缓冲液0.5mL,C、D组于术前7d分别给大鼠输注1×106个/只的供、受体致耐受DC。各组于术后第1、2、4、8周随机处死5只大鼠,进行移植牙病理学检查。结果炎性细胞浸润B组最重,A组最轻,C、D介于二者之间。牙根吸收A组最少,B组最多,C、D介于二者之间(P<0.05);2、4周时牙根吸收C、D组差异无统计学意义(P>0.05),在8周时D组低于C组(P<0.05)。第8周时牙周膜愈合点数C、D组少于A组,多于B组(P<0.05);炎性吸收B组最高(P<0.05);替代吸收A组最低(P<0.05)。结论两种不同来源的致耐受DC都可以降低牙移植的排斥反应,减少牙根的吸收,促进牙周膜的愈合;其受体致耐受DC更能减少牙根后期的吸收。

Objective To study the effects of different sources of dendritic cells （DCs） on root resorption and healing after allo-geneic tooth transplantation in rats .Methods The BN rats and Lewis rats were respectively used as the donor and recipient teeth for establishing the animal experimental transplantation models and randomly divided into 4 groups ,20 cases in each group ：the syn-geneic transplantation group（A） and the allogeneic transplantation groups（B ,C ,D） .The group A and B were infused with PBS buffer solution 0 .5 mL on 7 d before operation .The group C and D were infused with 1 × 106 donor or recipient tolerogenic DCs on 7 d before operation .5 rats randomly selected from each group were sacrificed for performing the pathological examinations of transplanted teeth at the end of 1 ,2 ,4 and 8 weeks .Results The inflammatory cellular infiltration was most severe in the group B , slightest in the group A and moderate in the group C and D .The root resorption was minimal in the group A ,maximal in the group B and moderate in the group C and D （P〈0 .05）;the root resorption at 2 ,4 weeks had no statistical difference between the group C and D（P〉0 .05） ,but which at 8 weeks in the group D was lower than that in the group C .The periodontal membrane healing points at 8 weeks in the group C and D was less than that in the group A ,but more than that in the group B （P〈0 .05）;the inflam-matory absorption was highest in the group B（P〈0 .05）;the alternative absorption was lowest in the group A （P〈0 .05） .Conclu-sion The two different sources of tolerogenic DCs all could reduce the rejection reaction of allogeneic tooth transplantation ,reduce the root resorption and promote the healing of periodontal membrane .Recipient tolerogenic DCs could reduce the root resorption in late stage even more .