J亚群禽白血病病毒PCR检测方法的构建与应用

Establishment and Application of PCR Method for Detecting Subgroup J Avian Leukosis Virus(ALV-J)

为建立简单、快速、准确、直接对疑似感染J亚群禽白血病病毒(Subgroup J of avian leukosis virus,ALV-J)鸡进行检测的PCR方法,根据GenBank中禽白血病病毒env基因序列设计1对特异性引物,构建了检测J亚群禽白血病病毒的PCR方法,并进行了特异性、敏感性试验和临床应用。结果表明:应用所构建的PCR方法对J亚群禽白血病病毒进行检测,其扩增结果与预期片段相符,对禽网状内皮细胞增生病病毒、鸡新城疫病毒和马立克氏病病毒的扩增结果均为阴性。说明,所构建的PCR检测方法具有较好的特异性和敏感性,可用于J亚群禽白血病病毒感染的检测、分子流行病学调查和分离毒株的快速鉴定。

The PCR method with simplicity,rapidity,accuracy and direction to detect suspected chicken infected with ALV-J was established based on one pairs of specific primers designed from the env gene sequence of ALV in GenBank and the PCR method was used in specific and sensitiveness tests and clinical application.The results showed that the amplification results of ALV-J by the established PCR method accord with the expected fragment and the amplification results of avian reticuloendothelial cell hyperplasia virus,Newcastle disease virus and Marek＆#39;s disease virus by the established PCR method all are negative,which indicates that the established PCR method with good specificity and sensitiveness can be used in detection of ALV-J infection,molecular epidemiological investigation and rapid identification of toxic strain separation.