不同的标本保存条件、溶血情况对髓过氧化物酶检测的影响

Effect of Different Specimen Storage Conditions and Hemolysis on Detection of Myeloperoxidase

目的 探讨同一患者不同的标本保存方式、保存时间、溶血情况对血液标本中髓过氧化物酶（MPO）含量的影响.方法 30例患者每人采集3管肝素钠抗凝静脉血,第一管30min内分离血浆,检验MPO含量做为基准值,其余两管分两组放常温（22℃）和2 ～8℃冰箱4h后检验,计算变化率（％）,组间采用配对t检验,评价常温和冰箱短时间内保存样本对MPO含量的影响;同时筛选不同MPO含量的15例患者,将其第一管血浆,分装保存于2～8℃和-20℃冰箱,分别于1天后、3天后、5天后测定MPO含量,计算下降率（％）,组间采用配对t检验,评价长时间内冰箱保存样本,冷藏和冷冻保存对MPO含量的影响.另外,对做完基准值的血样,人为造成溶血后测其MPO值,计算溶血的干扰偏差.结果 常温和冰箱短时间内保存4h,样本中MPO含量变化呈上升趋势,但差异无统计学意义（P＞0.05）.冷藏和冷冻的新鲜血浆样本长时间保存,MPO含量变化呈下降趋势,组间差异具有统计学意义（P＜0.01）,随着保存时间延长,含量下降越明显.标本不同程度的溶血均对测定MPO造成严重干扰.结论 MPO血浆样本采集后,要及时检验,在常温和2～8℃冰箱保存4h分离检测,对结果有影响,但影响较小,差异无统计学意义.分离新鲜血浆长时间冰箱保存,对结果影响差异有统计学意义,冷冻优于冷藏保存.不同程度的标本溶血均对测定MPO造成严重干扰.

Objective To explore the influence of different preservation methods, preservation time and hemolysis in the detection of myeloperoxidase （ MPO ）. Methods Blood samples were collected from the 30 patients. Everyone collected three blood samples, which were anticoagulated by heparin sodium. The first tube separated the plasma in 30 minutes, and then plasma MPO were assayed and considered as base line value. The remaining two samples were divided into two groups, one group was stored at the room temperature （22℃） for 4 hours, and the other was stored at refrigerator （2-8℃） for 4 hours. The MPO were detected. The first tube plasma of 15 patients were screened and respectively preserved in 2-8℃ and -20℃, according to which, the samples were divided into two groups. The MPO was assayed at 1,3 ,and 5 days. Results The MPO levels in samples stored at the room temperature and refrigerator at 4 hours was increase but without significant statistical differences in two groups （P 〉0.05）. The MPO levels in samples stored at 2-8℃ and -20℃ was decreased as the storage time prolong （P 〈 0.01 ）. The degrees of hemolysis had a substantial impact on analysis of MPO. ConclusionThe blood samples should be used for MPO detection immediately after sampling. The hemolysis has a substantial impact on analysis of MPO.