胶体金法和TPPA法检测血清梅毒抗体的比较与分析

Comparison of TPAb and TPPA Method for Detection of Serum Syphilis Antibodies

目的通过梅毒螺旋体明胶凝集试验(TPPA)和胶体金(Colloidal Gold,TPAb)检测血清梅毒抗体的比较,探讨胶体金法和TPPA法检测血清梅毒螺旋体抗体结果之间的关系。方法通过对本院首次用TPPA法检测阳性后的血清标本,再用TPAb法复查,共616例,以及急诊术前和腔镜检查前用TPAb法筛查出阳性的血清标本,再用TPPA法复查,共88例,并对7份两种方法共同阳性梅毒抗体血清用阴性血清进行倍比稀释,对稀释后的同一份血清分别用TPPA法和TPAb法进行定性检测,以比较两种方法灵敏度。结果 TPPA法检出616例阳性,用TPAb法复查612例阳,符合度99.35%;TPAb法共检测出88例阳性,用TPPA法复查77例阳性,符合度87.5%。结论稀释实验表明TPAb法灵敏度比TPPA法更高,能检测出更低的下限。

Objective To compare the TPAb and TPPA methods in detection of serum syphilis antibodies. Methods 616 cases of syphilis antibody positive serum samples measured by TPPA were repeat detected with TPAb method. 88 cases of syphilis antibody positive serum samples measured by TPAb were repeat detected with TPPA method. 7 syphilis antibody positive serum samples tested by both two methods were diluted with half negative serum were detected by TPPA and TPAb method respectively to compare the sensitivity of the two methods. Results 612 cases of serum samples in 616 TPPA tested positive cases were tested as positive by TPAb method,which showed 99.35% of compliance. 77 cases of serum samples in 88 TPAb tested positive cases were tested as positive by TPPA method, which showed 87.5 % of compliance. Conclusion The dilution experiment indicates that TPAb method is more sensitive than TPPA method, it can be used to detect samples with even lower limit.