摘要
目的检测慢性肾脏病(CKD)患者肾组织胸腺肽β4(Tβ4)的表达,并探讨其临床意义。方法选择广州市第一人民医院2010—2012年收治的CKD患者84例,根据肾小管间质纤维化分级标准分为0级组19例、Ⅰ级组25例、Ⅱ级组20例、Ⅲ级组20例;选择同期因创伤而在我院行肾切除术患者8例为对照组。收集所有患者肾组织标本,采用Envision免疫组化二步法检测Tβ4和α血管平滑肌肌动蛋白(α-SMA)的表达。结果对照组、0级组、Ⅰ级组、Ⅱ级组、Ⅲ级组Tβ4OD值分别为(0.21±0.01)、(0.25±0.03)、(0.30±0.03)、(0.33±0.02)、(0.36±0.04),α-SMA OD值分别为(0.22±0.02)、(0.24±0.01)、(0.29±0.04)、(0.35±0.02)、(0.39±0.04);对照组Tβ4OD值<0级组<Ⅰ级组<Ⅱ级组<Ⅲ级组(P<0.05),对照组与0级组α-SMA OD值<Ⅰ级组<Ⅱ级组<Ⅲ级组(P<0.05),而对照组α-SMA OD值与0级组比较,差异无统计学意义(P>0.05)。Pearson相关分析结果显示,肾小管间质纤维化程度与Tβ4表达水平、α-SMA表达水平均呈正相关(r=0.742、0.907,P<0.01);肾小管间质Tβ4表达水平与α-SMA表达水平亦呈正相关(r=0.767,P<0.01)。结论 Tβ4在肾小管间质纤维化的发生和发展过程中发挥着重要作用,可能参与了上皮细胞间质转分化(EMT)。
Objective To detect the expression of thymosinβ4in renal tissues in patients with chronic kidney disease（CKD）,to explore its clinical significance. Methods From2010 to 2012,a total of 84 CKD patients were selected,and then they were divided into 4 groups according to Renal Tubular Interstitial Fibrosis Grading Standard： 0- grade group（n = 19）,Ⅰ- grade group（n = 25）,Ⅱ- grade group（n = 20）,Ⅲ- grade group（ n = 20）. At the same time,8 cases underwent nephrectomy caused by trauma were selected as control group. Envision immunohistochemical two- step method was used to detect the expression of thymosinβ4and α- vascular smooth muscle actin（ α- SMA）. Results The OD of thymosinβ4of control group,0- grade group,Ⅰ- grade group,Ⅱ- grade group,Ⅲ- grade group was（0. 21 ± 0. 01）,（0. 25 ± 0. 03）,（0. 30± 0. 03）,（0. 33 ± 0. 02）,（0. 36 ± 0. 04）,respectively,and control group 0- grade group Ⅰ- grade group Ⅱ-grade group Ⅲ- grade group（P〈0. 05）; the OD of α- SMA of control group,0- grade group,Ⅰ- grade group,Ⅱ-grade group,Ⅲ- grade group was（0. 22 ± 0. 02）,（0. 24 ± 0. 01）,（0. 29 ± 0. 04）,（0. 35 ± 0. 02）,（0. 39 ± 0. 04）,respectively,and control group or 0- grade group 4 Ⅰ- grade group 4 Ⅱ- grade group 4 Ⅲ- grade group（ P〈0. 05）,but there was no significant difference between control group and 0grade group（ P〉0. 05）. Pearson correlation analysis showed that,renal tubular interstitial fibrosis grading was positively correlated with the expression of thymosin β4and α- SMA（r =0. 742,0. 907,P 0. 01）; the expression of thymosin β4was positively correlated with the expression of α- SMA（ r =0. 767,P 0. 01）. Conclusion Thymosin β4play an important role in the occurence and development of renal tubular interstitial fibrosis,may take participate in epithelial- mesenchymal transition（EMT）.
出处
《实用心脑肺血管病杂志》
2014年第9期31-34,共4页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
基金
2012年度广州市科技计划项目(2060404)
