溶菌酶稳定的银纳米粒子的合成及稳定性研究

Study on the preparation and stability of the lysozyme-stabilized silver nanoparticles

目的使用蛋白质做稳定剂来合成银纳米粒子,并探讨其稳定性,为其在生物医学领域及日常生活中的应用提供理论指导。方法采用化学还原法一步合成溶菌酶稳定的银纳米粒子,改变稳定性影响因素并观察银纳米溶胶的变化。即加入不同浓度的氯化钠来改变银纳米溶胶的离子强度,用酸或碱来调节溶胶pH,对溶胶进行多次冷冻-解冻循环。并且用柠檬酸稳定的银纳米粒子做对照试验。结果溶菌酶稳定的银纳米粒子对离子强度的变化比较敏感,在10 mmol/L NaCl条件下完全沉淀;在酸性条件下能够稳定存在(pH≤6);冷冻-解冻循环对其稳定性影响比较小。结论溶菌酶稳定的银纳米粒子的稳定性受周围环境影响较大,在使用及储存时需要加以注意。

Objective We have synthesized silver nanoparticles using protein as the stabilizer and discussed their stability to provide theoretical guidance for their application in biomedical field and daily life. Methods We have presented a one-step approach of chemical reduction to prepare the lysozyme-stabilized silver nanoparticles. Results The stability of lysozyme-stabilized silver nanoparticles was sensitive to the change of ionic strength and the nanoparticles precipitated completely in 10 mmol/L NaCI. The lysozyme-stabilized silver nanoparticles were stable under acidic condition （pH ≤6）. And the freeze-thawing cycle had a little influence on their stability. Conclusion The stability of lysozyme-stabilized silver nanoparticles is greatly influenced by the surrounding conditions. The variation caused by the stability factors should be considered for use and storage of the lysozyme-stabilized silver nanoparticles.