期刊文献+

HPLC法测定不同产地女贞叶中熊果酸的含量 被引量:4

Determination of the Content of Ursolic Acid in Folium Ligustrum lucidus from Different Habitats by HPLC
下载PDF
导出
摘要 [目的]用高效液相色谱法(HPLC)考察5个不同产地女贞叶中熊果酸的含量.[方法]用高效液相色谱法所用色谱柱为十八烷基硅烷键合硅胶色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-甲醇-0.5%醋酸铵溶液(67∶12∶21),检测波长为210 nm.[结果]方法学考察结果,回归方程为Y=4.715 5X+ 17.918,R2 =0.999 4,熊果酸含量在5.21~125.12 μg/ml浓度范围内线性关系良好.用该方法测得5个不同产地女贞叶中熊果酸的含量存在差异,其中上海市产的含量最高,为2.157 4 mg/g,南阳唐河产的含量最低,为0.863 0mg/g.[结论]同一采集时间,不同产地的女贞叶因环境因素,所含熊果酸的含量差异较大. [Objective]The content of ursolic acid in folium Ligustrum lucidus from different habitats was investigated by HPLC.[Method]Chromatographic column is eighteen alkyl silane bonded silica gel column chromatography (4.6 mm × 250 mm,5 μm),the mobile phase is acetonitrile-methanol-0.5 % ammonium acetate (67∶12∶21),the detection wavelength is 210 nm.[Result]Methodological study results:the regression equation:Y =4.715 5X + 17.918,R2 =0.999 4,the content of ursolic acid in 5.21-125.12 μg/ml,the concentration of the linear relationship is good.The content of ursolic acid in folium Ligustrum lucidus from different habitats existis differences,the highest content of Shanghai produced is up to 2.157 4 mg/g,the lowest content produced in Nanyang Tanghe is 0.863 0 mg/g.[Conclusion]At the same acquisition time,the content of ursolic acid in folim Ligustrum lucidus from different habitats has significant differences because of environmental factors.
机构地区 黄河科技学院
出处 《安徽农业科学》 CAS 2014年第28期9738-9739,共2页 Journal of Anhui Agricultural Sciences
基金 黄河科技学院校级立项(KYZR201311)
关键词 女贞叶 熊果酸 HPLC 含量测定 Ligustrum lucidus Ursolic acid HPLC Content determination
  • 相关文献

参考文献5

二级参考文献52

共引文献15

同被引文献48

引证文献4

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部