施用腐熟秸秆肥对烤烟成熟期碳代谢途径影响的初报

Preliminary report on effect of applying rotten straw fertilizer on carbon metabolism in maturing flue-cured tobacco

为了探明土壤添加腐熟秸秆对成熟期烤烟中部烟叶淀粉代谢途径的影响,明确获取优质烟叶的最佳施用量。本试验以烤烟品种NC89为试材,设置添加腐熟秸秆0 g/盆、80 g/盆和160 g/盆3个处理,研究不同量腐熟秸秆对成熟期烤烟淀粉积累、碳代谢相关酶活及关键基因表达的影响。结果表明:在烤烟发育成熟期,土壤添加不同量腐熟秸秆对中部叶还原糖、总糖及淀粉含量影响显著。土壤添加腐熟秸秆均可提高淀粉酶的活性,但是对转化酶影响不大;对碳代谢关键基因的表达分析表明添加腐熟秸秆增加了蔗糖合成酶基因(SS)、蔗糖转运蛋白基因(SUT1)、焦磷酸化酶基因(AGPase)和颗粒结合型淀粉合成酶基因(GBSSI)等关键基因的表达,从而促进成熟期烟叶碳代谢途径的进行,有利于提高烟叶的品质;其中每盆添加80 g腐熟秸秆取得的效果最好。

Tobacco variety NC 89 was sampled to investigate effect of applying rotten straw fertilizer on starch accumulation, enzymes activity of carbohydrate metabolism and expression of key genes in maturing flue-cured tobacco. Results showed that application of rotten straw fertilizer added in soil had significant impact on content of reducing sugar, total sugar and starch in middle tobacco leaves. It increased amylase activities while exerted little impact on invertase activities. It also increased expression level of starch biosynthesis-related genes and enzymatic activity ofSS, SUT1, AGPase, andGBSSI, thereby carbohydrate metabolism was facilitated.