HPLC-ELSD法同时测定复方三七漱口液中三七皂苷R_1、人参皂苷Rg_1、Rb_1的含量

Determination of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in compound Sanqi Gargle by HPLC-ELSD

目的 建立同时测定复方三七漱口液中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的HPLC-ELSD法.方法 采用DIKMA Diamonsil C18 （2）（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-水梯度洗脱,流速1.0 mL/min,柱温30℃,蒸发光散射检测器漂移管温度47℃,载气流速1.5 L/min.结果 三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1分别在0.41 ～1.53、1.55 ～ 5.83、1.58～5.92 μg呈良好线性关系;复方三七漱口液中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的平均回收率（n=9）分别为99.31％、100.30％、99.98％,RSD分别为1.60％、0.56％、0.97％.结论 该方法简便、准确,专属性、重复性好,可用于复方三七漱口液的质量控制.

Objective To establish a method for simultaneously determining notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in compound Sanqi Gargle by HPLC-ELSD.Methods A DIKMA Diamonsil C18 （2）column （250 mm × 4.6 mm,5 μm）was used with mobile phase of acetonitrile-water for notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1.The flow rate was 1.0 mL/min at 30 ℃.The ELSD drift tube temperature was 47 ℃ and carrier gas flow rate was 1.5 L/min.Results The linearities of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 were in the ranges of 0.41 ～ 1.53 μg,1.55 ～ 5.83 μg and 1.58 ～ 5.92 μg,respectively.The average recoveries （n =9）were 99.31％,100.30％,99.98％,respectively.Conclusion The method is simple,accurate,specificity,repeatability,and could be used for quality control of compound Sanqi Gargle.