共生真菌巴西类壳小圆孢中环二肽类代谢产物研究

Cyclic dipeptides metabolites from the symbiotic fungus paraconiothyrium brasiliense

利用正相和反相柱色谱,Sephadex LH-20凝胶柱色谱及反相HPLC等方法从昆虫共生真菌Paraconiothyrium brasiliense的发酵产物中分离得到9个环二肽类化合物,经NMR,MS及理化性质等方法,结构确定为环(L-亮氨酸-L-酪氨酸)(1),环(L-色氨酸-L-脯氨酸)(2),环(L-脯氨酸-L-苯丙氨酸)(3),环(L-脯氨酸-L-酪氨酸)(4),环(L-脯氨酸-L-亮氨酸)(5),环(L-亮氨酸-反式-4-羟基-L-脯氨酸)(6),环(L-异亮氨酸-L-脯氨酸)(7),环(L-缬氨酸-L-脯氨酸)(8),环(L-亮氨酸-L-异亮氨酸)(9)。所有化合物均为首次从该属菌种中分离得到。

Nine cyclic dipeptides secondary metabolites were isolated from the symbiotic fungus paraconiothyrium brasiliense, by normal phase silica gel,Sephadex LH-20 chromatography and reverse phase HPLC. Their structures were elucidated as cyclo-（ L-Leu-L-Tyr）（1）,cyclo-（L-Trp-L-Pro）（2）,cyclo-（L-Pro-L-Phe）（3）,cyclo-（L-Pro-L-Tyr）（4）,cyclo-（L-Pro-L-Leu）（5）,cyclo-（L-Leu-trans-4-hydroxy-L-Pro）（6）,cyclo-（L-Ile-L-Pro）（7）,cyclo-（L-Val-L-Pro）（8）,cyclo-（L-Leu-L-Ile）（9）by NMR,MS and physicochemical properties. All the compounds were isolated from the symbiotic fungus paraconiothyrium brasiliense for the first time.