荷移光度法测定注射用头孢唑啉钠含量

Determination of cefazolin sodium for injection based on charge-transfer reaction spectrophotometric method

以注射用头孢唑啉钠作为电荷给体,百里酚蓝作为电荷受体,用分光光度法研究了它们之间形成电荷转移配合物的条件。结果表明：在乙醇介质中,二者于室温即可形成1∶1的配合物,其最大吸收波长为450nm,4．67-93．32mg·L-1范围内呈线性关系,相关系数是0．9986,稳定常数是106．34,表观摩尔吸光系数ε=3．45＆#215;104 L·mol-1·cm-1。对形成配合物的机理进行了探讨。应用拟订的方法对注射用头孢唑啉钠进行了含量测定,结果与文献方法一致,平均回收率为99．9%。

Take cefazolin sodium for injection as donor,thymol blue as electron acceptor,charge transfer complexes formed between them was investigated by spectrophotometric method. The results show that 1：1 complexes can be formed at room temperature under ethanol medium with the maximum absorption wavelength 450 nm,linear range 4. 67-93. 32 mg·L-1 ,correlation coefficient 0. 9986, stability constant 106.34,apparent molar absorptivity ε=3. 45x10^4 L·mol-1·cm-1. The formation mechanism of complexes were dis-cussed. The results of the proposed method for determination of cefazolin sodium for injection are consistent with the literature repor-ted with average recovery rate 99. 9%.