AM真菌摩西球囊霉对盐胁迫条件下高羊茅生长特性的影响

Effects of arbuscular mycorrhizal fungus Glomus mosseae on the growth characteristics of Festuca arundinacea under salt stress conditions

随着全球变化,土壤次生盐渍化日益加重,直接影响到植物的生长发育。高羊茅是重要草坪绿化植物,如何提高其耐盐性是当前亟待解决的问题之一。本研究旨在前期试验的基础上,评价丛枝菌根(arbuscular mycorrhizal,AM)真菌改善高羊茅耐盐性的效应,探索AM真菌增强高羊茅耐盐性的作用机制。在盆栽条件下,对高羊茅接种摩西球囊霉并用不同浓度NaCl(0%,0.8%,1.2%和1.6%)溶液进行处理。结果表明,接种摩西球囊霉能增加高羊茅叶片抗氧化酶(SOD、POD和CAT)活性和抗氧化剂(抗坏血酸)含量、渗透调节物质(可溶性糖和脯氨酸)含量、矿质元素(N、P、S、Zn、K、Ca和Mg)含量、K/Na、Mg/Na、Ca/Na和植物内源激素脱落酸(ABA)、细胞分裂素(IPA)、吲哚乙酸(IAA)和赤霉素(GA)含量;降低丙二醛(MDA)、Na+、Cl-含量和膜透性。认为AM真菌能通过增强植物抗氧化系统的反应,降低氧化胁迫造成的伤害,减缓Na+和Cl-毒害,改善养分的吸收,强化渗透调节作用,维持激素平衡等来提高其耐盐能力。

As the global changes,the soil salinization becomes more and more serious and directly impacts plant growth.Festuca arundinacea is one of important lawn plants influenced with arbuscular mycorrhizal（AM）fungi and soil salinization.So how to improve the salt tolerance of F.arundinacea,is one of the urgent problems to be solved.The purpose of the present research was to evaluate the effects of AM fungi on improving salt tolerance of F.arundinacea,and to explore the mechanism of increasing salt tolerance of F.arundinacea by AM fungi.F.arundinaceaseeds were inoculated with AM fungus Glomus mosseae and treated with different NaCl concentration（0%,0.8%,1.2%and 1.6%）under pot conditions in a greenhouse.The activity of antioxidant enzymes（SOD,POD and CAT）,contents of antioxidant（AsA）,soluble sugar,proline,nutrient elements（N,P,S,Zn,K,Ca and Mg）,ABA（abscisic）,IPA（isopentenyl adenosine）,IAA（indole-3-acetic acid）,GA（gibberellin）and K/Na,Mg/Na,Ca/Na increased,in leaves of F.arundinacea plants colonized with G.mosseae,while the content of methane dicarboxylic aldehyde（MDA）,Na^＋and Cl^-decreased,as well as membrane permeability,compared to the noninoculation control.It was concluded that AM fungi could improve plant salt tolerance through improving nutrient absorption,osmotic regulation and the reaction of antioxidant system of plants,maintaining hormonal balance,reducing toxicity of Na^＋and Cl^-and damage caused by oxidative stress.