红鳍东方鲀SREBP-1基因的电子克隆及生物信息学分析

Silico cloning and bioinformatic analysis of SREBP-1 gene in torafugu Takifugu rubripes

利用斑马鱼的SREBP-1的氨基酸序列NP_001098599.1为探针,对红鳍东方鲀基因组数据库进行BLAST分析,获得红鳍东方鲀(Takifugu rubripes)的固醇调节元件结合蛋白-1(Sterol regulatory element binding protein-1,SREBP-1)的cDNA序列及相应的氨基酸序列。采用生物信息学方法对其基本理化性质、蛋白质结构及功能结构域进行分析。结果发现红鳍东方鲀SREBP-1基因的开放阅读框全长3 330 bp,共编码1 109个氨基酸;红鳍东方鲀SREBP-1的氨基酸序列与黄斑蓝子鱼、大西洋鲑、斑马鱼、人、牛及原鸡的同源性分别为81%、73%、72%、55%、55%、54%和54%;系统进化分析结果表明,红鳍东方鲀SREBP-1与黄斑蓝子鱼的进化关系最为密切,亲缘关系最近;红鳍东方鲀SREBP-1蛋白中α-螺旋较多,该蛋白质氨基端包含1个bHLH-zip的结构。

The fugu genome sequence database was screened with the amino acid sequence of zebrafish SREBP-1 （NP_001098599.1） as the probe on BLAST platform. We obtained a cDNA sequence encoding a putative （Sterol regulatory element binding protein-1, SREBP-1） of torafugu Takifugu rubripes and analyzed the deduced protein by bioinformatics methods in terms of its general physical and chemical properties, protein structures and functional domain structure. Sequencing and bioinformatics analysis showed that the full-length open reading frame of torafugu SREBP-1 cDNA consisted of 3 330 bp and encoded a polypeptide of 1 109 amino acids. The multiple sequence alignment showed that coding amino acid sequences of the obtained torafugu SREBP-1 gene had 81%, 73%, 72%, 55%, 55%, 54%, 54% homology with the corresponding Siganus canaliculatu, Salmo salar, Danio rerio, Homo sapien, Bos taurus, Gallus gallus, respectively. Phylogenetic tree analysis showed that torafugu SREBP-1 was closely related to Siganus canaliculatu SREBP- 1 than to those from other vertebrate species. After protein analysis, results showed that there was much more α-helix than other secondary structures in torafugu SREBP-1 protein, besides, it had a bHLH-zip domain in its N terminal.