指示植物检测甘薯病毒技术的改进研究

Improvement of Detected Technology of Test Viruses in Test-tube Plantlets of Sweet Potato by Indicator Plant

为了改进传统指示植物法检测甘薯病毒的技术,在已成功的巴西牵牛组织培养基础上,对巴西牵牛和甘薯试管苗茎段进行嫁接,培养,并检测甘薯病毒。结果显示,与带病毒的甘薯试管苗茎段嫁接的巴西牵牛,试管苗茎段叶片均能表现出黄化、花叶等阳性反应;与无病毒的甘薯试管苗茎段嫁接的巴西牵牛,试管苗茎段叶片均能健康生长。经5年对369个样本的检测,"甘薯、巴西牵牛试管苗嫁接法"和传统检测法对各个样本的检测结果完全一致。"甘薯、巴西牵牛试管苗嫁接法"可以替代传统的"巴西牵牛种子苗网室嫁接检测法"检测甘薯组培苗是否带有病毒,它是一种操作简单,灵敏度高,成本低,不受季节性和环境影响的新检测方法。

In order to improve the traditional technology of using phytoindicating to detect viruses on sweet potato, and on the basis of culturing tissue of lpomoea secosa successfully, the experiments were carried out for the detection of sweet potato viruses by grafting and culturing Ipomoea secosa and sweet potato cuttings in vitro. The results showed that when grafting with the virulifereus sweet potato cuttings in vitro, Ip- omoea setosa plantlets in vitro were positive as yellows or mosaic symptoms; and when grafting with non-viruliferons sweet potato cuttings in vitro, Ipomoea setasa plantlets in vitro growth was normal. By detecting 369 samples in 5 years, the results indicated that using sweet potato- Ipomoea secosa plantlets in vitro grafting technology was completely consistent with the traditional phytoindicating technology, which meant sweet potato-lpomoea setosa plantlets in vitro 8rafting technology could replace the traditional technology to detect virus on sweet potato tissue cultured seedlings. This new technique was proved to be high sensitivity, not influenced by outside environment, low cost, well-controlled culturing conditions and not restricted by seasons.