摘要
本文旨在研究家兔BMPR1B基因的多态性及其与生长速度的相关性。采用PCR产物测序方法检测BMPR1B基因编码区的多态性,利用PCR-RFLP技术对候选SNPs位点(c.117G>A)进行基因分型,研究其与家兔生长速度的关联性(N=262)。结果表明,在BMPR1B基因编码区共存在14个SNPs位点,它们分别是c.9C>T、c.54C>T、c.75T>A、c.88C>T、c.96T>C、c.117G>A、c.384A>G、c.392C>T、c.540C>T、c.603T>C、c.1254T>A、c.1332A>G、c.1356A>G及c.1436A>G,除c.392C>T突变致使该基因编码的第131个氨基酸亮氨酸(leu)被丝氨酸(ser)替换外,其他13个位点均为同义突变。c.117G>A位点处于Hardy-Weinberg平衡状态(P>0.05),为中度多态(0.25<PIC<0.50)。关联分析表明BMPR1B基因c.117G>A位点AA基因型35日龄断奶重显著高于GG基因型和AG基因型(669.556±43.733VS 612.571±10.588VS 612.478±13.73)。
In this paper, the aim of this experiment was to study the association between the polymorphisms of BMPRIB gene and body weight in rabbits. The polymorphisms of BMPRIB gene were detected by direct sequencing, the SNPs were genotypod by PCR-RFLP method, and the association between c. 117G 〉 A and body weight in 262 young New Zealand rabbis at different age stages was studied. The result showed that a total of 14 coding SNPs were found in BMPRI B gene. Among them, c. 9C 〉 T, c. 54C 〉 T, c. 75T 〉 A, c. 88C 〉 T, c. 96T 〉 C, c. ll7G〉A, c. 384A〉G, c. 54OC 〉T, c. 603T 〉 C, c. 1254T 〉 A, c. 1332A 〉G, c. 1356A 〉 G, and c. 1436A 〉G were synonymous SNPs, but c. 392C 〉 T was non-synonymous SNP which led leucine to serine. SNP of c. 117G 〉 A was at Hardy-Weinberg equilibrium （P 〉 0.05 ） and presented moderate po｜ymorphism （0.25 〈 PIC 〈 0.50）. The association analysis indicated that weight d BMPRI B gene AA 8euotype individuals weaning BW35 at c. 117G 〉 A site was significantly greater than that of GG and GA genotype individuals （ P 〈 0.05 ） （669.556 ±43.733VS 612.571± 10. 588VS 612.478 ± 13.73 ）.
出处
《西南农业学报》
CSCD
北大核心
2014年第4期1781-1785,共5页
Southwest China Journal of Agricultural Sciences
基金
国家兔产业技术体系建设(CARS-44-A-2)
