青蒿鳖甲汤加减对MRL/lpr狼疮鼠Th17细胞及肾脏病理的影响

Effect of Modified Qinghao Biejia Decoction on Th17 Cells and Renal Pathology of MRL/lpr Mice with Lupus Nephritis

【目的】探讨中药复方青蒿鳖甲汤加减对自发性红斑狼疮模型MRL/lpr小鼠Th17细胞及肾脏病理的影响。【方法】选用8~10周雌性MRL/lpr小鼠分为模型组、中药组、泼尼松组和联合用药组,各8只;8-10周龄雌性C57BL/6小鼠8只为正常对照组。中药组给予青蒿鳖甲汤加减水煎剂浓缩液（剂量为19.25 g·kg-1·d-1）,泼尼松组给予醋酸泼尼松片水溶液（剂量为8.75 mg·kg-1·d-1）,联合用药组同时给予上述2种药物,模型组和正常对照组给予生理盐水。给药7周后取小鼠脾脏及肾脏,采用流式细胞术检测脾脏单个核细胞悬液中Th17细胞的表达;光镜下观察小鼠肾脏病理改变并进行狼疮肾炎肾组织活动性指数（AI）评分。【结果】模型组小鼠Th17细胞比例显著高于正常对照组（P〈0.05）;各给药组Th17细胞比例均显著低于模型组（P〈0.05）,泼尼松组Th17细胞比例显著高于中药组（P〈0.05）。中药组、泼尼松组及联合用药组镜下均见肾脏病理改变明显较模型组轻,其狼疮肾炎肾组织活动性指标AI评分显著低于模型组（P〈0.05）。各组MRL/lpr小鼠AI评分与Th17细胞水平呈正相关（r=0.77,P〈0.01）。【结论】青蒿鳖甲汤加减可抑制MRL/lpr狼疮鼠Th17细胞的表达,可改善其狼疮肾炎肾组织活动性病理变化。

Objective To observe the effect of modified Qinghao Biejia Decoction （QBD） on Th17 cells and renal pathology of MRL/lpr mice with spontaneous systemic lupus erythematosus. Methods Thirty-two female MRL/lpr mice aged 8 to 10 weeks were divided into 4 groups： model group, Chinese medicine group, prednisone group, and combination group, 8 mice in each group. Eight female C57BL/6 mice aged 8 to 10 weeks served as normal control. Mice in Chinese medicine group were given concentrated solution of modified QBD （19.25 g·kg-1·d-1）, mice in the prednisone group were given water solution of prednisone acetate （8.75 mg·kg-1·d-1） , mice in the combination group were given the above two kinds of medicine, and mice in the model group and normal control group were given physiological saline. After medication for 7 weeks, spleens and kidneys in all of the groups were taken out for the experiment. Th17 cells in splenic mononuclear cell suspension were detected by flow cvtometrv, the pathological chanzes of renal tissue were observed under light microscope, and activity index （AI） of renal tissue in lupus nephritis mice was scored. Results The proportion of Th17 cells in the model group was significantly higher than that of normal control group （P〈0.05） . The proportion of Th17 cells in Chinese medicine group and combination group was lower than that of the model group （P〈0.05）, and prednisone group had higher proportion of Th17 cells than Chinese medicine group （P〈 0.05） . Compared with the model group, pathological changes of renal tissue were relieved, and AI scores were decreased in Chinese medicine group, the prednisone group and the combination group （P〈0.05） . Except for the normal control group , AI scores in all groups were positively correlated with the proportion of Th17 cells （r=0.77, P〈0.01） . Conclusion Modified QBD can inhibit the expression of Th17 cells and improve the pathological changes of MRL/lpr mice with lupus nephritis.