浙江省男男性接触HIV-1感染者病毒基因特征分析

Analysis on the genetic characteristics of HIV- 1 infected MSM individuals in Zhejiang province

目的分析浙江省2004—2011年117名男男性接触者(MSM)HIV-1感染病毒的序列特征,了解HIV-1毒株类型和特征。方法从实验室留样的MSM HIV感染者血液中提取DNA或RNA,用巢式PCR或RT-PCR方法扩增gag、pol基因区片段,测定序列并分析。结果 117名MSM HIV感染者中,序列结果按户籍来源覆盖21个省(市、自治区),感染毒株包括CRF01_AE 99名(84.62%)、B亚型7名(5.98%)、CRF07_BC 6名(5.13%)、CRF08_BC和CRF59_01B各1名(0.85%)。3名(2.56%)疑似01_B重组毒株与安徽感染者在系统进化树上聚集成可靠的次级进化簇(99%),且具有类似重组断点模式。84个CRF01_AE毒株的pol区基因序列聚集成簇,形成多个可靠的次级进化簇(30个节点的bootstrap值高于70%)。结论浙江省MSM HIV感染者主要流行CRF01_AE毒株,该人群HIV感染率高,应加强监测。CRF59_01B和新的01B重组毒株在浙江首次检出。

Objective To analyze the subtypes and characteristics of HIV- 1 among men who have sex with men（ MSM）in Zhejiang province from the year 2004 to 2011. Methods Blood DNA /RNA was extracted from the MSM HIV- 1infected individuals and then HIV- 1 gag and pol fragments were amplified by nested polymerase chain reaction（ nested-PCR） or RT- PCR. The positive PCR products were sequenced and the obtained sequences were analyzed by phylogenetic inference. Results A total of 117 HIV- 1 infected MSM individuals were analyzed,covering 21 provinces according to their household registry. Totally,three major strains were identified including CRF01_ AE（ 84. 62%,n = 99）,B（ 5.98%,n =7） and CRF07_BC（ 5.13%,n =6）. Moreover,3 novel 01 B recombinant stains,1 CRF59_01B and 1CRF08_BC were found. According to the neighbor- joining tree by pol fragments,there were more than 30 sub clusters with bootstrap value higher than 70% among the 84 CRF01_AE sequences. Conclusion CRF01_AE is the major HIV-1 infection strain among MSM in Zhejiang province. CRF59_01B and other novel 01 B recombinant stains are first reported among MSM individuals in Zhejiang province.