摘要
目的探讨S100A9在小鼠脑缺血再灌注损伤中的表达及其意义。方法将Toll样受体4(TLR4)基因突变型小鼠C3H/HeJ(n=21)和TLR4野生型C3H/HeN小鼠(n=21)分别随机分为野生型对照组(C3H/HeN对照,n=8),突变型对照组(C3H/HeJ对照,n=8),野生型模型组(C3H/HeN模型,n=13)以及突变型模型组(C3H/HeJ模型,n=13),采用线栓法建立大脑中动脉栓塞模型,于再灌注12h后行神经功能缺损评分;TTC染色测定脑梗死体积;苏木精-伊红染色评价脑组织损伤程度;免疫荧光成像和实时定量PCR技术检测S100A9蛋白及mRNA表达情况。结果C3H/HeJ模型组神经缺损评分低于C3H/HeN模型组;脑梗死体积和神经细胞损伤程度也较C3H/HeN模型组减轻;C3H/HeJ和C3H/HeN模型组梗死侧脑组织中S100A9mRNA和蛋白较各自对照组表达均明显增高,且C3H/HeJ模型组脑组织中S100A9表达量较C3H/HeN模型组明显减少。提示在脑缺血再灌注损伤时S100A9蛋白表达与TLR4关系密切。结论 S100A9有可能通过活化TLR4信号通路在脑缺血再灌注损伤过程中发挥重要作用。
Objective To investigate the expression of S100A9 in cerebral ischemia-reperfusion injury and it significance.Methods Toll-like receptor 4（TLR4）gene mutant mice C3H/HeJ（n=21）and TLR4wild-type mice C3H/HeN（n=21）were randomly divided into four groups,which were C3H/HeN control group（n=8）,C3H/HeJ control group（n=8）,C3H/HeN model group（n=13）and C3H/HeJ model group（n=13）.The model of focal ischemia was established by transient middle cerebral artery occlusion（MCAO）.After reperfusion for 12 h,scoring for neurological deficit was performed;the brain infarcted size was evaluated by TTC staining;the brain damage was assessed by HE staining;S100A9expression was detected by immunofluorescence staining and real-time PCR,respectively.Results The neurological deficit score was much lower in the C3H/HeJ model group than in C3H/HeN model group.The brain infarcted size and the brain damage were significantly attenuated in C3H/HeJ model group when compared with those in C3H/HeN model group.The mRNA and protein expression of S100A9 was significantly increased in C3H/HeJ model group and C3H/HeN model group as compared with that in their control groups.It was much lower in C3H/HeJ model group than in C3H/HeN model group.It was suggested that the expression of S100A9 protein was closely associated with TLR4 in cerebral ischemia-reperfusion injury.Conclusion S100A9 plays an important role in cerebral ischemia-reperfusion injury by activating the TLR4 signaling pathway.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2014年第4期417-420,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.81201444
No.81101401)
