人尿激肽原酶对脑缺血再灌注大鼠缺血灶TGF-β1表达的影响

Effects of human urinary kallidinogenase on TGF-β1 expression in rats with focal cerebral ischemia-reperfusion

目的观察人尿激肽原酶对大鼠脑缺血再灌注损伤缺血灶转化生长因子-β1(transforming growth factor,TGF-β1)表达的影响,探讨人尿激肽原酶对脑缺血再灌注损伤的保护机制。方法实验大鼠随机分为对照组(sham组)、缺血再灌注组(model组)和用药组(test组)。通过线栓法制作大鼠大脑中动脉脑缺血再灌注模型,用药组于再灌注后5 min静脉注射人尿激肽原酶。在缺血2 h再灌注48 h后断头取脑,检测缺血灶脑组织TGF-β1的表达。结果与缺血再灌注组相比,用药组缺血灶脑组织TGF-β1的表达明显增高,差异有统计学意义(P<0.05)。结论人尿激肽原酶对脑缺血再灌注损伤的保护作用,其机制可能与促进TGF-β1的表达有关。

Objective To observe the effects of human urinary kallidinogenase on the expression of transforming growth factor（ TGF-β1 ）after ischemia-reperfusion injury in rats. Method Rats were randomly assigned into three groups： sham operation group（ sham group） , focal cerebral ischemia-reperfusion group（ model group） and human urinary kallidinogenase group （test group）. The middle cerebral artery occlusion reperfusion model was made by the suture method （ischemia for 2 hours,and reperfusion for 48 hours）. Rats were decapitated to detect the TGF-β1 expression in the infarct area. Results Compared with model group,the expression of TGF-β1 in brain tissues was increased in test group （P 〈 0.05）. Conclusion Human Urinary kallidinogenase can relieve the brain damage partly by promoting TGF-β1 synthesis in brain.