水中SaV GⅣ的提取及荧光定量PCR检出方法研究

Extraction of SaV GⅣ in water sample and real-time QPCR detection method

札幌病毒(Sapovirus/SaV)是引起急性腹泻的常见病毒,水体是SaV传播的主要途径之一。由于设备和检测水平的限制,水体中病毒的检出费时费力。本文利用分子诊断学中灵敏度高的水中病毒富集技术结合荧光定量PCR方法,对水中SaV GⅣ基因型快速检测方法进行了研究。获得了一套水中病毒富集、核酸提取及分子诊断学检出方法。对比了TaqMan探针法与SYBRGreen染料法在检测中的效果。初步建立了利用水中病毒分离装置富集SaV GⅣ病毒,应用TaqMan探针法与SYBRGreen染料法两种荧光定量PCR方法快速检出SaV GⅣ的分子诊断学方法。

Sapovirus（ SaV） is a common cause of gastroenteritis. Water is one of the main media for the propagation of SaV. Because of a limitation in equipment and inspection ability,the detection of SaV in water is laborious. In this study,molecular diagnostics with highly sensitive nucleic acid extraction technology and a real-time quantitative PCR method were used for the rapid detection of SaV GⅣ in water. A rapid detection method for SaV GⅣ in water has been established.