毛细管电泳测定乌头类药材中6种生物碱的含量

Simultaneous Determination of Six Alkaloids in Aconitum Plants by HPCE

目的 建立高效毛细管电泳法（HPCE）同时测定乌头类药材中乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱和苯甲酰新乌头原碱含量的方法。方法 以40 mmol·L-1乙酸钠-60 mmol·L-1乙酸铵-80 mmol·L-1tris试剂-25%甲醇（pH 8.3）为缓冲溶液,未涂渍标准熔融石英毛细管（75μm×64.5 cm,有效长度56 cm）为分离通道,分离电压为16 kV,检测波长为235 nm,毛细管温度为25℃,压力进样为5 kPa×6 s。结果 6种生物碱的浓度与峰面积的线性关系良好（r〉0.999 7）;加样回收率为95.25%~103.91%。用此法测定了乌头类药材中6种生物碱的含量,得到满意结果。结论 该方法简单、准确,重复性较好,可用于乌头类药材内在质量的评价和控制。

OBJECTIVE To establish a high performance capillary electrophoresis （HPCE） method for simultaneous determina- tion of aconitine, hypaconitine, mesaconitine, benzoylaconine, benzoy]hypaconine and benzoylmesaconine in the herbs from Aconitum plants. METHODS The solution of 40 mmol · L-lsodium acetate-60 mmol · L-1 ammonium acetate-80 mmol · L-1 Tris-25% meth- anol was used as buffer solution （ pH 8.3 ） , uncoated fused silica capillary （64. 5 cm· 75 μm id, 56 cm of effective length） was used for separation, the separation voltage was 16 kV, detection wavelength was set at 235 nm, column temperature was maintained at 25 ℃, and sample was injected at 5 kPa × 6 s. RESULTS Six alkaloids showed good linearity （ r 〉 0. 999 7） in the ranges of the tested concentrations, and the average recoveries of the method were between 95.25% - 103.91%. CONCLUSION The method is sim- ple, accurate and reproducible, and can be used for quality control of the herbs from Aconitum plants.