豆甾二烯用于特级初榨橄榄油掺假检测的研究

Study on stigmastadienes used for extra virgin olive oil adulteration detection

研究了利用豆甾二烯进行特级初榨橄榄油的掺假检测,考察了在特级初榨橄榄油中掺入不同比例油橄榄果渣油、榛子油、葵花籽油、大豆油、玉米油、花生油、米糠油、棕榈油和核桃油,采用高效液相色谱法(HPLC)测定掺和油中的3,5-豆甾二烯含量。植物油样品用石油醚溶解,过硅胶柱净化,C30柱分离,乙腈/叔丁基甲醚(75∶25,V/V)作流动相,紫外检测波长235nm。结果表明,本方法具有良好的回收率及精密度,3,5-豆甾二烯是特级初榨橄榄油掺假鉴别的一个十分重要的特征性指标,本方法能够鉴别出了特级初榨橄榄油中掺入0.3%油橄榄果渣油、0.5%榛子油、2.1%葵花籽油、0.5%大豆油、0.3%玉米油、1.5%花生油、0.2%米糠油、0.6%棕榈油和0.6%核桃油。

The stigmastadienes used for extra virgin olive oil adulteration detection was investigated. Stigmasta3,5-diene content of the extra virgin olive oil blended with different proportion of olive pomace oil, hazelnut oil, sunflower oil ,soybean oil ,corn oil ,peanut oil, rice bran oil, palm oil and walnut oil were detected by highperformance liquid chromatography （HPLC）. Vegetable oil samples were dissolved with petroleum ether, cleaned up with a silica gel column,separated with C30 column and determined by ultraviolet detection at 235nm. Acetonitrile/tert-butyl methyl ether（75：25,V/V） was served as mobile phase in isocratic elution. The results showed that the method had good recovery and precision,and stigmasta-3,5-diene was an important characteristic index which could be used for extra virgin olive oil adulteration detection. The proposed method could reliably identify extra virgin olive oil blended with 0.3% olive pomace oil ,0.5% hazelnut oil,2.1% sunflower oil,0.5% soybean oil,0.3% corn oil, 1.5% peanut oil,0.2% rice bran oil ,0.6% palm oil and 0.6% walnut oil, respectively.