大孔树脂富集发酵大豆提取物中乙酰胆碱酯酶抑制剂的工艺研究

Study on enrichment of acetylcholinesterase inhibitor from fermented soybean with macroreticular resin

为了确定适宜的大孔树脂型号及富集乙酰胆碱酯酶抑制剂的最佳工艺条件,通过静态吸附率和解吸液抑制率的测定,确定较优树脂型号,并考察动态解吸的相关影响因素。实验结果表明:ADS-17为富集乙酰胆碱酯酶抑制剂的适宜树脂,其静态吸附率和解吸液抑制率分别可达96.65%、64.24%,其适宜富集工艺条件:静态吸附饱和的树脂用80%乙醇溶液进行动态解吸,洗脱速度为1.0mL/min时,解吸液AChE抑制率为62.64%。

To determine the appropriate type of macroporous resin and optimum conditions of enriching acetylcholinesterase inhibitors. Optimum type of resin was determined by measuring the rate of static adsorption and desorption fluid inhibition,and the dynamic desorption＇s related factors was studied. Results indicated that ADS-17 was the suitable resin on enriching acetylcholinesterase inhibitors,and the rate of static adsorption and desorption fluid inhibition could reach 96.65%,64.24%. The optimum enrichment conditions were as follows： macroreticular resin by static adsorption saturation,elution solvent of 80% ethanol,elution rate of 1.0mL/min. Under the mentioned conditions,the desorption fluid AChE inhibition rate reached 62.64%.