汉中油菜花黄色素微波辅助提取及抗氧化活性研究

Study on the extraction and anti-oxidative activities of yellow pigment with microwave-assisted extraction from Hanzhong rapeseed flowers

以汉中油菜花为原料,采用微波助提对所含黄色素进行提取,利用正交设计实验优化提取工艺,考察色素对食用油的抗氧化活性、对羟自由基及过氧化氢的清除能力。结果表明,油菜花黄色素的最佳提取工艺条件是:95%乙醇为提取溶剂,微波时间为30s,水浴温度为80℃,料液比为1∶40(g/mL),回流时间为15min。抗氧化实验表明,油菜花中所含黄色素具有较强的抗氧化能力,可抑制植物油氧化。在一定的浓度范围内,色素对羟自由基及过氧化氢的抗氧化活性随浓度升高而增强。

The pigment was extracted from rapeseed flowers by microwave-assisted extraction technology. On the basis of single-factor experiments,the optimal operating conditions were obtained with orthogonal design. The optimum extraction conditions was established as follows ：the concentration of ethanol 95%,extracted 30s with microwave radiation,the reflux time was 15min,the reaction temperature was 80℃ and the ratio of material to liquid 1：40. The yellow pigment could inhibit the oxidant degree of the edible oil. The capacity to scavenge hydroxyl free radical and H2O2 increased as well as the concentration within a certain degree.