摘要
以栀子提取物缓释片为模型药物,栀子苷为成分指标,DPPH自由基清除率为抗氧化活性指标,对以药效指标评价中药缓释制剂的可行性进行探讨研究。通过建立可计量的体外DPPH测定方法,以该方法测定栀子缓释片不同时间点体外释放液的抗氧化活性,绘制时间-效应曲线,采用线性回归和f_2相似因子法与栀子苷的时间-累积释放度曲线比较,结果显示体外释放度评价中DPPH药效指标与栀子苷成分指标有较好相关性,提示DPPH清除率药效指标可以用来评价以环烯醚萜类为主要成分的栀子缓释片的体外释放行为。
Using sustained release tablets of gardenia extract as model drugand DPPH radical scavenging capacity as antioxi- dant index, the feasibility of using pharmacodynamics index was explored to evaluate sustained release tablets. Applying the established quantifiable method of DPPH radical scavenging to the dissolved liquid of model drug, release profiles and biological effects profiles were drawn, and their correlation was discussed. A good correlation was observed by linear regression and f2 factor, suggesting that the indicator could be used to evaluate sustained release tabletsofextracts of gardenia in which iridoids were mainly involved.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第17期3274-3277,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81102816)
国家重点基础研究发展计划(973)项目(2012CB724001)
北京中医药大学科研创新团队项目(2011-CXTD-13)
关键词
栀子
缓释
DPPH自由基
药效指标
gardenia
sustained release
DPPH radical
pharmacodynamics index