穿心莲内酯对白念珠菌生物膜分散的影响

Effect of andrographolide on Candida albicans biofilm dispersion

随着真菌感染日渐增多,白念珠菌的防治成为当前抗真菌感染的重点。该研究拟探讨中药单体穿心莲内酯(AG)对白念珠菌生物膜分散的影响。实验以微孔板结合医用导管片构建白念珠菌生物膜模型,分别通过XTT减低法和平板法发现1 000,500,250 mg·L^(-1)AG能不同程度的影响白念珠菌生物膜分散细胞的活性;以扫描电镜观察导管片残余生物膜形态结构,发现1 000,500,250 mg·L^(-1)AG能剂量依赖性的诱导白念珠菌生物膜的分散,且分散出的细胞以酵母相为主;实时荧光定量PCR(qRT-PCR)法检测发现AG使HSP90表达上调,使UME6和PES1表达下调。该研究表明AG能一定程度的诱导白念珠菌生物膜分散。

Along with the increase in fungal infections, Candida albicans prevention and control become the focus of anti-fun- gal infection at present. This study aims to discuss the effect monomer andrographolide （AG） on C. albicans biofilm dispersion. In the experiment, micro-well plates and medical catheter pieces were used to establish the C. albicans biofilm model. It was discovered by XTT assay and flat band method that 1 000, 500,250 mg · L-1 AG could impact the activity of C. albicans biofilm dispersion cells. The morphological structures of residual biofilms on catheter pieces were observed with scanning electron microscopy, which showed that 1 000, 500,250 mg·L-1 AG could induce C. albicans biofilm dispersion in a dose-dependent manner, and the dispersed cells were dominated by the yeast phase. According to the real-time fluorescence quantification PCR （qRT-PCR） test, AG could up-regulate HSP90 expression and down-regulate UME6 and PES1 expressions. This study demonstrates that AG could induce C. albicans biofilm dispersion to some extent.