排石颗粒特征图谱研究

Study on Fingerprints of Paishi Granule

目的:研究建立排石颗粒多味药特征图谱鉴别方法。方法:采用UPLC-G2 Q-Tof液质联用系统进行色谱分离和色谱峰归属,以十八烷基硅烷键合硅胶为填充剂;以0.1%甲酸溶液为流动相A,以甲醇:乙腈(2:1)为流动相B,以一定梯度洗脱,流速:0.2ml/分钟;检测波长为240nm。结果 26个色谱峰得到较好分离,初步归属15个色谱峰。方法的精密度、重复性、稳定性良好。结论:本方法可同时鉴别排石颗粒6味药材,可用于排石颗粒质量控制。

Objective： To establish characteristic chromatograms of 6Chinese herbal medicines in Paishi Granule by using UPLC-G2Q-Tof-MS. Methods： The analysis was carried out on ACQUITY UPLC BEH C18 （2. lmmxl00mm, 1.71xm）, the detection wavelength was 240ram. The gradient elution was 0.1% formic acid （A） and methanol-acetonitrile （2 ： 1 ） （ B）, the flow rate was 0. 2ml . min-1. Results： 26peaks were found in the characteristic chromatograms of Paishi Granule and 15peaks of them were prehminarily indentified. Precision, reproducibility and stabihty is all right. Conclusion： The method can identify 6Chinese herbal medicines and can be used to control the quality of Paishi Granule.