通气培养对小球藻生长及生物组成的影响

Effect of aeration cultivation on the growth and biological component of Chlorella sp.

采用小球藻进行了静置培养和通气培养试验,研究了通气条件对小球藻生长速度、生物量、生物组成及脂肪酸组成的影响。结果表明:相对于静置培养(不通气),通气培养条件下,小球藻在稳定期的生长速度、生物量及其单位产量上高出10倍以上,差异极显著;小球藻的蛋白质含量、总糖含量明显较高,而总脂含量、淀粉含量则较低;小球藻饱和脂肪酸含量显著降低,不饱和脂肪酸特别是多不饱和脂肪酸含量升高。不同通气量对小球藻的生长也存在差异,其较适宜的通气条件为1L/min。在小球藻的培养过程中,可通过适宜的通气措施来改善培养效果和获得较高的目标产物。

Chlorella sp. was cultured under stationary condition and aeration condition respectively. The effects of aeration on the growth rate, biomass, biological component and fatty acid composition of Chlo-rella sp. were studied. The results showed that comparing with stationary cultivation, the growth rate, bi-omass and productivity of Chlorella sp. in stationary phase under aeration condition were ten times high-er; the contents of protein and total sugar increased significantly, while the contents of total lipid and starch reduced; the saturated fatty acids became less, while unsaturated fatty acids, especially polyunsat-urated fatty acids went up; different aerations had different effects on the growth of Chlorella sp. , and the suitable aeration condition was 1 L/ min. The proper aeration measure could be used to improve cultiva-tion efficacy and obtain higher desired product during the cultivation of Chlorella sp. .