基于HPLC-DAD多波长数据的蓝色圆珠笔墨水鉴别(英文)

Discrimination of blue ballpoint pen inks using multiwavelength data of HPLC-DAD

利用HPLC-DAD多波长数据分析鉴别不同品牌的蓝色圆珠笔墨水.使用HPLC-DAD分离68支不同品牌的蓝色圆珠笔中油墨的染料组分,并用HPLC-MS方法确认主要染料组分的化合物结构.根据检测到的主要染料组分,将所有的蓝色圆珠笔油墨分成9类,进一步利用主成分分析方法,处理多波长色谱数据对同一类别中不同品牌的墨水进行区分.实验结果表明,多波长的色谱数据比单一波长对蓝色圆珠笔墨水更具鉴别能力.组合2步鉴别过程,对于蓝色圆珠笔品牌的鉴别率可达94%.

In this paper, muhiwavelength data of HPLC-DAD were used to discriminate different brands of blue ballpoint pen ink. Dyes from inks of sixty-eight blue ballpoint pens were analyzed by using HPLC-DAD. Dye components detected in the inks were further identified by using HPLC-mass spectrometry （HPLC-MS）. All the inks were firstly classified into nine groups according to the presence or absence of the significant chromatographic peaks. Then the inks in the same group were further discriminated by using PCA with muhiwavelength chromatographic data. The results indicated that chromatographic data extracted from three wavelengths were more potent in the discrimination as compared with a single wavelength chromatographic data. By combining the two discrimination steps, we can discriminate about 94% of blue ballpoint pens according to the brands.