盐酸埃克替尼对ACC-M细胞MMP-2、E-cadherin蛋白表达的影响

Influence of Icotinib Hydrochloride upon the Protein Expression of ACC-M Cells MMP-2 and E-cadherin

盐酸埃克替尼是一种表皮生长因子受体酪氨酸激酶抑制剂,利用免疫细胞化学方法和间接免疫荧光联合流式细胞技术,检测它在体外对人涎腺腺样囊性癌细胞株(ACC-M)MMP-2、E-cadherin蛋白表达的影响.结果表明:盐酸埃克替尼可降低MMP-2蛋白表达,增加E-cadherin蛋白表达,可抑制人涎腺腺样囊性癌ACC-M细胞的浸润、转移.

Icotinib hydrochloride is a kind of epidermal growth factor receptor tyrosine kinase inhibitor,whose influ-ence upon the protein expression of ACC-M cells MMP-2 and E-cadherin can be tested by way of immunocytochem-istry and the technique of indirect immunofluorescence together with flow cytometry. The results show that icotinib hydrochloride can reduce the protein expression of MMP-2,but increase that of E-cadherin,thus it can be used to restrain the infiltration and metastasis of ACC-M cells.