蓝莓夏普蓝组培苗繁殖技术研究

Research on Propagation of Tissue-culture Seedlings of Blueberry “Xiapulan”

以蓝莓"夏普蓝"品种为试验材料研究了组培苗繁殖技术。在培养基中添加不同浓度ZT、蔗糖,分析了其对组培苗丛生芽诱导增殖的影响,以及不同IBA浓度对组培苗生根的影响。结果表明:改良WPM+ZT 1.25 mg/L培养条件下,"夏普蓝"组培苗丛生芽分化效果最佳。在培养基中附加蔗糖是蓝莓增殖培养所必需的,蔗糖浓度过高或过低,都可能对蓝莓增殖产生不良影响,以蔗糖40 g/L时的增殖效果最好;生长素IBA对"夏普蓝"的生根诱导有促进作用,IBA 0.6 mg/L对诱导蓝莓组培苗生根最有效,生根率可达88%,生根速度较快,粗细均匀,根系壮。

The propagation techniques of tissue - culture seedlings of blueberry variety ＂Xiapulan＂ were studied. The influences of various media containing different concentrations of ZT and sucrose on the induced propagation of cluster buds of the tissue - culture seedlings, as well as the effects of different concentrations of IBA on the rooting of the tissue - culture seedlings were analyzed. The results showed that the differentiation of cluster buds of ＂Xiapulan＂ tissue - culture seedlings was the best on the im- proved WPM ＋ ZT 1.25 mg,/L medium. It was necessary for the propagation of blueberry to add sucrose into the medium, but overhigh or too low concentration of sucrose could cause adverse effect on the propagation of blueberry, and 40 g/L sucrose had the best effect. The auxin IBA had promotive action on the rooting of tissue - culture seedlings of ＂Xiapulan＂, and 0.6 mg/L IBA had the best rooting effect： the rooting rate reached 88%, the rooting speed was relatively high, and the roots were strong and uniform in thickness.