洗涤厂排污口可培养中度嗜热菌的多样性

Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory

为考查洗涤厂热废水排放口土壤在常年人为热环境下的可培养嗜热菌的多样性,采集洗涤厂热废水排放口附近的湿热泥土,应用不同培养基分离纯化泥土中的可培养嗜热菌,并对获得的纯培养细菌进行16SrDNA鉴定和系统发育相关分析。结果表明:分离纯化得到的29株不同表型的纯培养菌株均属于坚壁菌门芽孢杆菌纲,归属于5个属11个种,分别是地芽孢杆菌属Geobacillus 3个种,芽孢杆菌属Bacillus 3个种,无氧芽孢杆菌属Anoxybacillus 3个种,解脲芽孢杆菌属Ureibacillus 1个种和高温放线菌属Thermoactinomyces 1个种,属种多样性丰富,菌株资源具有潜在开发应用价值。

To investigate the diversity of mesophilic bacteria resulting from constant thermal conditions , the soil sample was collected at outfall of thermal wastewater from laundry factory .The culturable mesophilic bacteria were isolated with different medium to obtain pure culture , identified by 16S rDNA and the data were analyzed in phylogenetic tree .The results showed that the 29 strains in pure culture were obtained from the thermal wastewater outfall soil with different phenotype .The strains were classified into Bacilli ,attributed to 5 genus and 11 species , including three species of Geobacillus ,three species of Bacillus ,three species of Anoxybacillus ,one species of Ureibacillus ,and one species of Thermoactinomyces .The diversity of species was rich ,and the resources of species would have potential in practical application .