刮痧对腰椎间盘突出症大鼠血清IgG及椎间盘组织病理改变的影响

Effects of Gua Sha therapy on IgG in Serum and Pathological Changes of Disc Tissue of Rats with Lumbar Disc Herniation

目的 探讨刮痧对腰椎间盘突出症模型大鼠的作用机理.方法 将50只雄性SD大鼠随机分为空白组、假手术组、模型组、刮痧组、药物组,每组10只.空白组不做任何处理;假手术组只做假手术处理;模型组、刮痧组和药物组进行造模：通过手术将大鼠尾椎髓核移植于L4～L5处左侧肌肉层中.模型组不给予干预措施;于术后第5天,刮痧组给予刮痧,药物组给予药物硫唑嘌呤（3 mg/kg）灌胃.分别于造模前1d、造模后第4天（干预前）、干预后第6天、干预后第12天、干预后第18天（干预结束后）采血.应用双抗体两步夹心酶联免疫吸附法（ELISA）检测血清中免疫球蛋白G（IgG）的含量.干预后第18天（干预结束后）采血完成后处死大鼠.空白组、假手术组切取相应尾椎椎间盘,模型组、刮痧组、药物组取出移植尾椎椎间盘,并在光镜下观察尾椎椎间盘的病理变化.结果 与模型组相比,刮痧组和药物组的血清IgG含量明显降低,差异有统计学意义（P＜0.01）,而刮痧组与药物组之间没有显著性差异（P＞0.05）,提示刮痧和药物都可以抑制造模引起的自身免疫反应,降低模型大鼠血清IgG值,且效果相当.椎间盘组织病理切片显示,假手术组无明显的炎症反应,刮痧组和药物组椎间盘周围组织局部免疫炎症反应与模型组相比明显减轻.结论 刮痧可以抑制大鼠髓核引起的自身免疫及免疫炎症反应.

OBJECTIVE To explore the mechanism of Gua Sha therapy on the model rats with lumbar disc herniation （LDH）.METHODS Fifty male SD rats were divided into 5 groups randomly：blank group,sham operation group,model group,Gua Sha group and drug group （n =10 each group）.The rats of blank group did not receive any treatment,of sham operation group with sham operation,of model group,Gua Sha group and drug group were modeled：transplant nucleus pulposus of rat＇s coccygeal vertebra disc to the left muscle layer near L4～L5 by operation,among which model group were given no intervention; Gua Sha group were given Gua Sha therapy on the 5th day after operation; Drug group were fed with azathioprine through intragastric gavage （3 mg/kg）,followed by drawing blood on the day before operation,the 4th day after operation （the day before treatment）,the 6th day after interventions,the 12th day after interventions and the 18th day after interventions （the end of treatment） and detecting the IgG in serum by ELISA method.After the last blood drawing,all rats were killed.The corresponding coccygeal vertebra disc of the rats in blank group and sham operation group and the transplanted coccygeal vertebra discs in model group,Gua Sha group,and drug group were taken out to observe pathological changes of coccygeal vertebra disc under optical microscope.RESULTS Compared with the model group,serum level of IgG of Gua Sha group and drug group were obviously decreased,with significant difference （P〈0.01）,but without statistical significance between the two groups （P〉0.05）,suggesting that both Gua Sha therapy and drug could inhibit the autoimmunity reaction caused by modeling,with similar effects on lowering serum level of IgG in model rats.The pathological slice of intervertebral tissue showed that there was no obvious inflammation reaction in sham operation group,and the topical immune-inflammatory reaction of intervertebral peripheral tissue in Gua Sha group and drug group was obviously alleviated.CONCLUSION Gua Sha therapy could inhibit the autoimmunity and immuno-inflammatory reaction caused by transplantation of nucleus pulposus.