活血通腑方抗实验性肠粘连作用及机制研究

Study on Experimental Anti-adhesion Effect of Huoxue Tongfu Fang and the Mechanism

目的 观察活血通腑方对肠粘连的治疗效果,探讨其对肠粘连防治作用的机理.方法 取SD雄性大鼠60只,随机分为6组：对照组、模型组、活血通腑方高、中、低剂量组、地塞米松磷酸钠阳性对照组.除对照组外,其余各组大鼠均制备肠粘连模型.对照组和模型组腹腔注射生理盐水,地塞米松组腹腔注射地塞米松磷酸钠（10mg/kg）,活血通腑方低、中、高剂量组灌胃给予活血通腑方（0.65、1.3、2.6 g/100 g）,连续给药1周.各组于术后第8天取血,ELISA法测定血清IL-6、IL-1β和TNF-α含量;取粘连组织,匀浆后试剂盒检测TGF-β1、CTGF的含量,同时记录大鼠肠粘连级别.RT-PCR检测粘连组织中FN mR-NA的表达.Western blot检测p-Smad3的蛋白水平.结果 模型组血清IL-1β和TNF-α含量增高,FN mRNA的表达增加,TGF-β1、CTGF含量增高.与模型组相比,活血通腑方低、中、高剂量组的肠粘连程度明显减轻,血清IL-1β和TNF-α及粘连组织中TGF-β1和CTGF含量明显下降（P＜0.05～0.01）,FN mRNA和p-Smad3表达降低（P＜0.05～0.01）.结论 活血通腑方可减轻肠粘连的程度,对肠粘连具有防治作用.

OBJECTIVE 60 male SD rats were randomly divided into six groups：normal control group,model group,low,medium and high dose of Huoxue Tongfu Fang groups and dexamethasone sodium phosphate positive control group.All groups were prepared for adhesion models except normal control group; control group and model group were injected with saline; dexamethasone group received intraperitoneal injection of dexamethasone sodium phosphate （10 mg/kg） ; low,medium and high doses of Huoxue Tongfu Fang groups orally administered Huoxue Tongfu Fang （0.65,1.3,2.6 g/100 g） a week for all.On the 8th day,blood in each group was taken out and IL-6,IL-1β and TNF-α in serum were determined in ELISA method; adhesion tissue was taken and TGF-β1 and CTGF levels were detected with ELISA kit after homogenate; adhesion degree was recorded.The expression of FN mRNA in adhesion tissue was tested in RT-PCR; p-Smad 3 protein level was detected in Western blot.RESULTS IL-1β and TNF-α in model group increased; so did the expression of FN mRNA,TGF-β1 and CTGF levels.Compared with model group,the degree of adhesion in all doses of Huoxue Tongfu Fang significantly reduced; IL-1β and TNF-α in serum and TGF-β1 and CTGF levels significantly declined （P〈0.05～0.01）; the expression of FN mRNA and pSmad 3 decreased （P〈0.05～0.01）.CONCLUSION Huoxue Tongfu Fang can reduce the degree of adhesion,making a preventive and therapeutic effect on adhesion.