植物总RNA提取研究进展

Research Progress of Isolation of Plant Total RNA

植物总RNA的提取尽管已成为一种常规的技术,但由于不同植物化学组分的差异,没有一种特定的方法适用于所有植物RNA的提取。该文介绍了异硫氰酸胍法、改进CTAB法、改进SDS法和Trizol试剂盒法提取植物总RNA的特点。

Although the isolation of total RNA has become a common method,there is not yet an ideal method for the isolation of total plant RNA ：lue to the different chemical compositions of plant.The guanidinium thiocyanate method, modified CTAB method, modified SDS method and total RNA extraetion kit of Trizol were introduced to isolate high purity and integrity RNA from plant.