油桐种子脂肪酸延长代谢的转录表达与代谢调控机理研究

Analysis of Fatty Acid Elongation Metabolism Pathways Based on the Transcriptome in Tung Oil Tree Seeds

油桐是我国四大木本油料植物之一,当前对油桐的研究主要集中在栽培选优及低产林改造方面,而对油桐脂肪酸延长代谢的分子机理研究尚未见报道。本研究以油桐果实膨大期、油脂转化初期和油脂转化高峰期的种子为材料,采用高通量RNA测序技术对油桐种子脂肪酸延长代谢的3个不同时期转录组进行比较,以nr、Swiss-Prot、KEGG和COG 4个蛋白数据库为参考,对油桐种子脂肪酸延长代谢进行了综合性分析。研究结果表明,参与油桐种子脂肪酸延长代谢途径的非冗余基因序列共37条,涉及的主要酶功能基因有9种;在果实膨大期、油脂转化初期和油脂转化高峰期中,调控脂肪酸延长代谢途径的基因存在明显的表达差异。综合分析结果绘制了油桐种子脂肪酸延长代谢途径,揭示了调控油桐种子脂肪酸延长代谢过程的基因作用规律。这些研究结果为油脂合成的遗传改良提供了资源和技术基础,同时为油桐分子设计育种提供了一定的科学依据。

Tung oil tree（ Vernicia fordii） is one of the four woody oil plants in China. The current research of tung oil tree primarily focuses on selective breeding,cross-breeding,and reconstruction of low-yield forests,but the molecular mechanisms related to fatty acid elongation are still not covered. RNA-seq technology was used to compare and analysis the different expression of tung oil tree＇s fatty acid elongation metabolism during three different development stages,including fruit enlargement stage,initial stage of lipid synthesis,and peak stage of lipid synthesis.This research comprehensively analyzed the fatty acid elongation metabolism in tung oil tree＇s seeds based on the nr,Swiss-Prot,KEGG,and COG database. The results showed that there were 37 non-redundant sequences participating in fatty acid elongation metabolism,which involving 9 kinds of main enzyme genes. Through the transcriptome data analysis,the genes regulating fatty acid elongation metabolism had different expression in tung oil tree＇s seeds during three different development stages. And fatty acid elongation metabolism pathway was drawn according the results of comprehensive analysis. Meanwhile,this research revealed the mechanism of regulatory genes in fatty acid elongation metabolism pathway of tung oil tree＇s seeds. These results not only provide resources and technical infrastructure for genetic improvement in tung oil tree＇s fatty synthesis but also provided some scientific basis for molecular breeding of tung oil tree.