特早熟甘蓝型油菜异交率的测定

Determination of Early Maturing Brassica napus L. Outcrossing Rate

利用SSR分子标记技术构建3个特早熟甘蓝型油菜恢复系的指纹图谱,筛选出这3个恢复系的共显性SSR标记,测定了这3个恢复系的异交率。结果表明,恢复系材料4395、3509、4152的异交率分别为46.02%、33.32%、18.12%,在P<0.01时呈极显著差异,说明这3个恢复系的异交差异明显,为确定这3个恢复系在综合杂交种中的比例提供依据。

Fingerprinting of three very early matured Brassica napus restorer lines was conducted by using SSR molecular markers. Codominant SSR markers were found in the 3 restorer lines and their F1. Those primers were used to determine the outcrossing rate of these 3 restorer lines in fields. The results showed that the outcrossing rate of these restorer lines（ 4395,3509,and 4152） were respectively 46. 02%,33. 32%,and 18. 12%. There was a significant difference at 0. 01 level,which illustrated that the outcrossing rate of three restorer lines had differences.And it provided a basis to determine the proportion of three restorer lines in synthetic hybrids.