盐胁迫下豌豆幼苗对内外源NO的生理生化响应

Physiological and biochemical response of pea seedlings to endogenous and exogenous NO under salt stress

以豌豆品种"陇豌一号"为材料,用NO供体硝普钠、NO清除剂c-PTIO及硝普钠类似物亚铁氰化钠处理豌豆幼苗,研究了内外源NO对盐胁迫下豌豆幼苗生长的影响。结果表明:添加外源NO能明显使盐胁迫下豌豆幼苗胚芽和胚根长、幼苗干重、根冠比及叶绿素含量增加(P<0.05),可溶性蛋白和可溶性糖含量升高,豌豆幼苗叶片SOD、POD和CAT活性增加(P<0.05),叶片质膜相对透性降低,还能减缓MDA含量的上升并提高脯氨酸含量。内源NO对盐胁迫下豌豆幼苗胚芽生长、幼苗干重、可溶性糖含量、MDA含量以及SOD、CAT活性均具有明显的调节作用。因此,内外源NO均可缓解盐胁迫对豌豆幼苗的伤害。

A pea variety ‘longwan1 ’was used as the experimental material to study the effect of endogenous and exogenous NO on the growth of pea seedlings under NaCl stress with NO donor sodium nitroprusside （SNP）,NO scavenger c-PTIO and sodium nitroprusside analogues sodium ferrocyanide.It was found that adding exogenous NO increased the plumule and radicle length,seedling dry weight,root-shoot ratio,chloro-phyll content,soluble protein and sugar levels,and the activities of SOD,POD and CAT of the pea seedlings. The content of proline was also increased under salt stress.Meanwhile,exogenous NO also alleviated the accu-mulation of MDA and decreased the membrane relative permeability in leaves of pea seedlings under salt stress. Endogenous NO had a significant effect on growth of germ,seedling dry weight,soluble sugar content,and MDA,SOD and CAT activity.In summary,exogenous and endogenous NO both alleviated the damage to pea seedlings caused by salt stress.