放线菌D01对马铃薯4种病原真菌的抑菌作用

Antagonistic effect of actinomycetes D01 against four strains potato pathogens

采用PDA平板对峙法、生长速率法等方法,研究了放线菌D01对马铃薯干腐病菌、马铃薯黑痣病菌、马铃薯炭疽病菌和马铃薯早疫病菌4种病原真菌的抑菌活性。结果表明,放线菌D01对4种病原真菌均表现出显著的抑菌活性,发酵液抑菌率均在70%以上;经放线菌处理后,病原真菌的基内菌丝不同程度的发生了畸变;放线菌D01对供试靶标真菌均有显著的拮抗作用,其中对马铃薯干腐病菌和马铃薯黑痣病菌的持续抑菌作用最强。

Fungistatic activity of actinomycetes strain D01 on Fusarium spp.,Rhizoctoniasolani,Colletotri-chumcoccodesand Alternariasolaniwas studied by confronting incubation on PDA plates,and assessing the fungi growth rates.An inhibition ratio of more than 70% to four potato fungal pathogens was gained by D01 . Substrate mycelium of target pathogens suppressed with antagonistic actinomycetes had been deformed or lysed.Actinomycetes D01 showed persistent inhibition effects on the target pathogens in different degrees,es-pecially on C.coccodesand R.solani.